[The ratio of fungi and bacteria in the biomass of different types of soil determined by selective inhibition].

Tundra, chernozem (virgin and arable), sod-podzolic (coniferous forest, meadow, and arable), and grey forest (larch forest) soils were used to separate the contributions of fungi and bacteria to substrate-induced respiration (SIR) with the help of antibiotics. For soils with a high content of organic matter (tundra and chernozem: 12 and 8%, respectively), the procedure of selective inhibition of SIR has been optimized. The optimized procedure includes the application of high concentrations of streptomycin (50-120 mg/g of soil) and cycloheximide (50-80 mg/g of soil) and decreasing the weight of the analyzed soil sample. Soils under study have shown the predominant contribution of fungi (63-82%) to the total SIR. The fungal-bacterial ratio in the soils of natural ecosystems (0-5 cm, without litter) was 4.32, 2.19, 1.5, and 1.5 for tundra soil, virgin chernozem, coniferous (sod-podzolic soil), and larch (grey forest soil) forests, respectively. The lower layers of sod-podzolic (5-10 cm) and grey forest (48-58 cm) soils showed a decrease in the fungal and increase in the bacterial component in the total SIR.