Literature DB >> 1720442

A monoclonal antibody sandwich immunoassay for serum amyloid A (SAA) protein.

T L McDonald1, A Weber, J W Smith.   

Abstract

An antibody sandwich immunoassay using two purified rat monoclonal antibodies to human serum amyloid A was developed and used to measure serum amyloid A in human serum. The assay was specific, sensitive, reproducible, and reliable and does not require denaturation of the specimen prior to assay. Serum amyloid A purified by hydrophobic interaction chromatography of acute phase human serum afforded a reliable standard for the assay. A significant (r = 0.69) correlation for SAA and C reactive protein values was found for 180 patient samples analyzed.

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Year:  1991        PMID: 1720442     DOI: 10.1016/0022-1759(91)90081-p

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  17 in total

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Authors:  Helle G Olsen; Kerstin Skovgaard; Ole L Nielsen; Páll S Leifsson; Henrik E Jensen; Tine Iburg; Peter M H Heegaard
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10.  Milk amyloid A as a biomarker for diagnosis of subclinical mastitis in cattle.

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