Jeffrey H Ware1, Zhaozong Zhou, Levy Kopelovich, Ann R Kennedy. 1. Department of Radiation Oncology, Division of Oncology Research, University of Pennsylvania School of Medicine, 3620 Hamilton Walk, Philadelphia, PA 19104-6072, USA. jhware@mail.med.upenn.edu
Abstract
BACKGROUND: The successful use of clonal selection through fluctuation analysis of human cancer cells as a means for studying tumor progression has been previously reported. MATERIALS AND METHODS: Three clones derived from a parental population of human prostate cancer (LNCaP) cells were selected based on proliferation, hormone sensitivity and anchorage-independent growth. The effects of five potential cancer preventive agents were evaluated using cell proliferation, anchorage-independent growth and apoptosis as end-points. RESULTS: Clone 21 cells, which represent a presumptive normal phenotype, were generally more sensitive than Clone 17 and Clone 6 cells, which represent a more malignant phenotype, to fluasterone, 7beta-HF, L-selenomethionine and troglitazone in assays for proliferation and/or apoptosis. CONCLUSION: The results confirm the efficacy of the above agents as cancer chemopreventive agents and support our contention that clonal selection of established human cancer cells provides a model to study the efficacy of chemopreventive agents.
BACKGROUND: The successful use of clonal selection through fluctuation analysis of humancancer cells as a means for studying tumor progression has been previously reported. MATERIALS AND METHODS: Three clones derived from a parental population of humanprostate cancer (LNCaP) cells were selected based on proliferation, hormone sensitivity and anchorage-independent growth. The effects of five potential cancer preventive agents were evaluated using cell proliferation, anchorage-independent growth and apoptosis as end-points. RESULTS: Clone 21 cells, which represent a presumptive normal phenotype, were generally more sensitive than Clone 17 and Clone 6 cells, which represent a more malignant phenotype, to fluasterone, 7beta-HF, L-selenomethionine and troglitazone in assays for proliferation and/or apoptosis. CONCLUSION: The results confirm the efficacy of the above agents as cancer chemopreventive agents and support our contention that clonal selection of established humancancer cells provides a model to study the efficacy of chemopreventive agents.
Authors: Jelena Stewart; Jeffrey Ware; Cecilie Boysen; Sandeep Gulati; Zhaozong Zhou; Simon Rosenfeld; Levy Kopelovich; Ann R Kennedy Journal: Nutr Cancer Date: 2008 Impact factor: 2.900