BACKGROUND: Procathepsin D (pCD) secreted by cancer cells, increases proliferation, metastasis and progression of breast cancer, however its role in lung cancer is still unclear. The purified pCD and its synthetic activation peptide (AP) have shown similar proliferative effect on various cancer cell lines. The aim of this study is to clarify the role ofpCD and its AP in lung cancer by stable expression of pCD and pCD lacking its AP, in NCI-H23 lung cancer cells. MATERIALS AND METHODS: The stable transfected clones were tested for cell proliferation, invasion and growth in nude mice. The effect of exogenous addition of purified pCD and its mutant proteins was also analyzed by proliferation assay. RESULTS: The invasion and proliferation in vitro and tumor growth in vivo, demonstrated that the expression of pCD enhances the carcinogenic properties of NCI-H23 cells and that the AP is essential for these activities. Exogenous addition of purified proteins on various lung cancer cell lines showed that neither catalytic activity nor glycosylation are involved in the growth-promoting activity. CONCLUSION: This is the first report of pCD cDNA expression in lung cancer cells that enhances the growth and invasion of these cells both in vitro and in vivo.
BACKGROUND: Procathepsin D (pCD) secreted by cancer cells, increases proliferation, metastasis and progression of breast cancer, however its role in lung cancer is still unclear. The purified pCD and its synthetic activation peptide (AP) have shown similar proliferative effect on various cancer cell lines. The aim of this study is to clarify the role ofpCD and its AP in lung cancer by stable expression of pCD and pCD lacking its AP, in NCI-H23 lung cancer cells. MATERIALS AND METHODS: The stable transfected clones were tested for cell proliferation, invasion and growth in nude mice. The effect of exogenous addition of purified pCD and its mutant proteins was also analyzed by proliferation assay. RESULTS: The invasion and proliferation in vitro and tumor growth in vivo, demonstrated that the expression of pCD enhances the carcinogenic properties of NCI-H23 cells and that the AP is essential for these activities. Exogenous addition of purified proteins on various lung cancer cell lines showed that neither catalytic activity nor glycosylation are involved in the growth-promoting activity. CONCLUSION: This is the first report of pCD cDNA expression in lung cancer cells that enhances the growth and invasion of these cells both in vitro and in vivo.
Authors: Victoria J Butler; Wilian A Cortopassi; Andrea R Argouarch; Sam L Ivry; Charles S Craik; Matthew P Jacobson; Aimee W Kao Journal: J Mol Biol Date: 2019-01-25 Impact factor: 5.469
Authors: Daniel E Abbott; Naira V Margaryan; Jacqueline S Jeruss; Seema Khan; Virginia Kaklamani; David J Winchester; Nora Hansen; Alfred Rademaker; Zhila Khalkhali-Ellis; Mary J C Hendrix Journal: Cancer Biol Ther Date: 2010-01-15 Impact factor: 4.742
Authors: N S Vasudev; S Sim; D A Cairns; R E Ferguson; R A Craven; A Stanley; J Cartledge; D Thompson; P J Selby; R E Banks Journal: Br J Cancer Date: 2009-10-06 Impact factor: 7.640