Literature DB >> 17200552

Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.

Greg B G Moorhead1, Timothy A J Haystead, Carol MacKintosh.   

Abstract

Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.

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Year:  2007        PMID: 17200552     DOI: 10.1385/1-59745-267-X:39

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  5 in total

Review 1.  Affinity-based profiling of endogenous phosphoprotein phosphatases by mass spectrometry.

Authors:  Brooke L Brauer; Kwame Wiredu; Sierra Mitchell; Greg B Moorhead; Scott A Gerber; Arminja N Kettenbach
Journal:  Nat Protoc       Date:  2021-09-13       Impact factor: 13.491

2.  A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors.

Authors:  Kali A Smolen; Arminja N Kettenbach
Journal:  J Vis Exp       Date:  2022-04-29       Impact factor: 1.424

3.  Conserved residues in the N terminus of lipin-1 are required for binding to protein phosphatase-1c, nuclear translocation, and phosphatidate phosphatase activity.

Authors:  Bernard P C Kok; Tamara D Skene-Arnold; Ji Ling; Matthew G K Benesch; Jay Dewald; Thurl E Harris; Charles F B Holmes; David N Brindley
Journal:  J Biol Chem       Date:  2014-02-20       Impact factor: 5.157

4.  A Quantitative Chemical Proteomic Strategy for Profiling Phosphoprotein Phosphatases from Yeast to Humans.

Authors:  Scott P Lyons; Nicole P Jenkins; Isha Nasa; Meng S Choy; Mark E Adamo; Rebecca Page; Wolfgang Peti; Greg B Moorhead; Arminja N Kettenbach
Journal:  Mol Cell Proteomics       Date:  2018-09-18       Impact factor: 5.911

5.  Displacement affinity chromatography of protein phosphatase one (PP1) complexes.

Authors:  Greg B G Moorhead; Laura Trinkle-Mulcahy; Mhairi Nimick; Veerle De Wever; David G Campbell; Robert Gourlay; Yun Wah Lam; Angus I Lamond
Journal:  BMC Biochem       Date:  2008-11-10       Impact factor: 4.059

  5 in total

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