A Krisanapiboon1, B Buranapanitkit, K Oungbho. 1. Department of Orthopaedic and Physical Medicine, Faculty of Medicine, Prince of Songkla University, Haadyai, Songkhla, Thailand.
Abstract
PURPOSE: To investigate the biocompatibility of hydroxyapatite composite (hydroxyapatite, plaster of Paris, and chitosan) impregnated with gentamicin, fosfomycin, imipenem, or amphotericin B. METHODS: The interactions of the extract from each drug against osteoblast were tested using the methylthiotetrazole test. RESULTS: Extracts from all drugs showed good biocompatibility at concentrations varying from 10 microgram/ml to 1000 microgram/ml. Imipenem and amphotericin B at a concentration of 1000 microgram/ml had a significantly higher percentage of cell viability than the control group. No morphological change of osteoblast was observed in all drug tests at any concentrations. CONCLUSION: The hydroxyapatite composite had a good biocompatibility for carrying gentamicin, fosfomycin, imipenem, or amphotericin B.
PURPOSE: To investigate the biocompatibility of hydroxyapatite composite (hydroxyapatite, plaster of Paris, and chitosan) impregnated with gentamicin, fosfomycin, imipenem, or amphotericin B. METHODS: The interactions of the extract from each drug against osteoblast were tested using the methylthiotetrazole test. RESULTS: Extracts from all drugs showed good biocompatibility at concentrations varying from 10 microgram/ml to 1000 microgram/ml. Imipenem and amphotericin B at a concentration of 1000 microgram/ml had a significantly higher percentage of cell viability than the control group. No morphological change of osteoblast was observed in all drug tests at any concentrations. CONCLUSION: The hydroxyapatite composite had a good biocompatibility for carrying gentamicin, fosfomycin, imipenem, or amphotericin B.
Authors: Miriam Miranda; Adolfo Fernández; Sonia Lopez-Esteban; Francisco Malpartida; José S Moya; Ramón Torrecillas Journal: J Mater Sci Mater Med Date: 2012-04-18 Impact factor: 3.896