Literature DB >> 1719357

Rapid method for isolation and staining of bacterial lipopolysaccharide.

A al-Hendy1, P Toivanen, M Skurnik.   

Abstract

Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.

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Year:  1991        PMID： 1719357     DOI： 10.1111/j.1348-0421.1991.tb01562.x

Source DB:  PubMed          Journal:  Microbiol Immunol        ISSN： 0385-5600            Impact factor:   1.955


  11 in total

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