BACKGROUND: Mastocytosis is a heterogeneous group of disorders characterized by abnormal accumulation of mast cells. OBJECTIVE: Skin biopsies from 24 patients (23 with proven mastocytosis) were screened for the presence of the c-kit Asp-816-Val mutation. METHODS: In frozen biopsies, RNA was isolated, cDNA synthesis and PCR, the expected PCR product of 346 bp was obtained from 23 patients. RESULTS: In patients with urticaria pigmentosa, the mutation was detected in 38% of the adults and 25% of the children. With regard to the clinical presentation of the disease, no difference was found between adult patients with and without the mutation, as detected with our assay. One out of the 2 children with the mutation had an atypical presentation of the disease. CONCLUSION: the mutation could not be detected in all the patients, probably due to lack of sensitivity of the methods.
BACKGROUND:Mastocytosis is a heterogeneous group of disorders characterized by abnormal accumulation of mast cells. OBJECTIVE: Skin biopsies from 24 patients (23 with proven mastocytosis) were screened for the presence of the c-kitAsp-816-Val mutation. METHODS: In frozen biopsies, RNA was isolated, cDNA synthesis and PCR, the expected PCR product of 346 bp was obtained from 23 patients. RESULTS: In patients with urticaria pigmentosa, the mutation was detected in 38% of the adults and 25% of the children. With regard to the clinical presentation of the disease, no difference was found between adult patients with and without the mutation, as detected with our assay. One out of the 2 children with the mutation had an atypical presentation of the disease. CONCLUSION: the mutation could not be detected in all the patients, probably due to lack of sensitivity of the methods.
Authors: Melody C Carter; Sarah T Clayton; Hirsh D Komarow; Erica H Brittain; Linda M Scott; Daly Cantave; Donna M Gaskins; Irina Maric; Dean D Metcalfe Journal: J Allergy Clin Immunol Date: 2015-06-01 Impact factor: 10.793
Authors: Carlos A Torres-Cabala; Wei-Lien Wang; Jonathan Trent; Dan Yang; Su Chen; John Galbincea; Kevin B Kim; Scott Woodman; Michael Davies; Jose A Plaza; J W Nash; Victor G Prieto; Alexander J Lazar; Doina Ivan Journal: Mod Pathol Date: 2009-08-28 Impact factor: 7.842
Authors: Remko Hersmus; Hans Stoop; Gert Jan van de Geijn; Ronak Eini; Katharina Biermann; J Wolter Oosterhuis; Catharina Dhooge; Dominik T Schneider; Isabelle C Meijssen; Winand N M Dinjens; Hendrikus Jan Dubbink; Stenvert L S Drop; Leendert H J Looijenga Journal: PLoS One Date: 2012-08-28 Impact factor: 3.240