OBJECTIVE: The confocal laser scanning microscope represents an effective tool for studying biological samples stained for fluorescence observation. In this study we have used the confocal microscope to analyze ghost cells in calcifying cystic odontogenic tumors. STUDY DESIGN: Specimens from 15 calcifying cystic odontogenic tumor cases were stained with hematoxylin and eosin, and scanned by a confocal laser scanning microscope to generate optically sectioned images. RESULTS: All of the analyzed samples presented autofluorescent cells that were identified as ghost cells. The degree of autofluorescence intensity was variable and may be a result of the presence of hard keratin. CONCLUSION: The confocal laser scanning microscope may be of help in analyzing and defining the nature and extent of keratinization processes in calcifying cystic odontogenic tumor ghost cells.
OBJECTIVE: The confocal laser scanning microscope represents an effective tool for studying biological samples stained for fluorescence observation. In this study we have used the confocal microscope to analyze ghost cells in calcifying cystic odontogenic tumors. STUDY DESIGN: Specimens from 15 calcifying cystic odontogenic tumor cases were stained with hematoxylin and eosin, and scanned by a confocal laser scanning microscope to generate optically sectioned images. RESULTS: All of the analyzed samples presented autofluorescent cells that were identified as ghost cells. The degree of autofluorescence intensity was variable and may be a result of the presence of hard keratin. CONCLUSION: The confocal laser scanning microscope may be of help in analyzing and defining the nature and extent of keratinization processes in calcifying cystic odontogenic tumor ghost cells.
Authors: Dardo Menditti; Luigi Laino; Marina DI Domenico; Giuseppe Troiano; Mario Guglielmotti; Sara Sava; Antonio Mezzogiorno; Alfonso Baldi Journal: In Vivo Date: 2018 Sep-Oct Impact factor: 2.155
Authors: C H Siar; Toshiyuki Kawakami; R R Buery; K Nakano; M Tomida; H Tsujigiwa; P P Han; H Nagatsuka; K H Ng Journal: Eur J Med Res Date: 2011-11-10 Impact factor: 2.175
Authors: Dario Di Stasio; Marco Montella; Antonio Romano; Giuseppe Colella; Rosario Serpico; Alberta Lucchese Journal: Cancers (Basel) Date: 2022-01-23 Impact factor: 6.639