Literature DB >> 1718770

A dot-blot hybridization procedure for the detection of astrovirus in stool samples.

M M Willcocks1, M J Carter, J G Silcock, C R Madeley.   

Abstract

We have developed a nucleic acid dot-blot hybridization test for the detection of astroviruses in stool samples. The test was not as sensitive as electron microscopy for the detection of low numbers of well preserved astrovirus particles, but was able to identify astroviruses in stools containing particles of indistinct morphology. In total, this procedure identified astroviruses in more samples than did electron microscopy, and the data indicate that the incidence of astroviruses may be substantially underestimated.

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Year:  1991        PMID： 1718770      PMCID： PMC2272075          DOI： 10.1017/s0950268800049049

Source DB:  PubMed          Journal:  Epidemiol Infect        ISSN： 0950-2688            Impact factor:   2.451


  11 in total

1.  Growth and characterisation of human faecal astrovirus in a continuous cell line.

Authors:  M M Willcocks; M J Carter; F R Laidler; C R Madeley
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

2.  Diagnosis of astrovirus gastroenteritis by antigen detection with monoclonal antibodies.

Authors:  J E Herrmann; N A Nowak; D M Perron-Henry; R W Hudson; W D Cubitt; N R Blacklow
Journal:  J Infect Dis       Date:  1990-02       Impact factor: 5.226

3.  Human astrovirus serotypes.

Authors:  J B Kurtz; T W Lee
Journal:  Lancet       Date:  1984-12-15       Impact factor: 79.321

4.  Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies.

Authors:  J E Herrmann; R W Hudson; D M Perron-Henry; J B Kurtz; N R Blacklow
Journal:  J Infect Dis       Date:  1988-07       Impact factor: 5.226

5.  An electron microscopical investigation of faecal small round viruses.

Authors:  A R Oliver; A D Phillips
Journal:  J Med Virol       Date:  1988-02       Impact factor: 2.327

6.  The electron microscopical and physical characteristics of small round human fecal viruses: an interim scheme for classification.

Authors:  E O Caul; H Appleton
Journal:  J Med Virol       Date:  1982       Impact factor: 2.327

7.  "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1984-02       Impact factor: 3.365

8.  Letter: 28 nm particles in faeces in infantile gastroenteritis.

Authors:  C R Madeley; B P Cosgrove
Journal:  Lancet       Date:  1975-09-06       Impact factor: 79.321

9.  Six-year retrospective surveillance of gastroenteritis viruses identified at ten electron microscopy centers in the United States and Canada.

Authors:  J F Lew; R I Glass; M Petric; C W Lebaron; G W Hammond; S E Miller; C Robinson; J Boutilier; M Riepenhoff-Talty; C M Payne
Journal:  Pediatr Infect Dis J       Date:  1990-10       Impact factor: 2.129

10.  A 27-nm virus isolated during an outbreak of acute infectious nonbacterial gastroenteritis in a convalescent hospital: a possible new serotype.

Authors:  L S Oshiro; C E Haley; R R Roberto; J L Riggs; M Croughan; H Greenberg; A Kapikian
Journal:  J Infect Dis       Date:  1981-06       Impact factor: 5.226
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  6 in total

1.  Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses.

Authors:  S S Monroe; B Jiang; S E Stine; M Koopmans; R I Glass
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103

2.  Prevalence of human astrovirus serotype 4: capsid protein sequence and comparison with other strains.

Authors:  M M Willcocks; J B Kurtz; T W Lee; M J Carter
Journal:  Epidemiol Infect       Date:  1995-04       Impact factor: 2.451

3.  Prevalence of human astrovirus serotypes in the Oxford region 1976-92, with evidence for two new serotypes.

Authors:  T W Lee; J B Kurtz
Journal:  Epidemiol Infect       Date:  1994-02       Impact factor: 2.451

4.  Annual incidence, serotype distribution, and genetic diversity of human astrovirus isolates from hospitalized children in Melbourne, Australia.

Authors:  E A Palombo; R F Bishop
Journal:  J Clin Microbiol       Date:  1996-07       Impact factor: 5.948

Review 5.  Outbreaks of food-borne and waterborne viral gastroenteritis.

Authors:  C W Hedberg; M T Osterholm
Journal:  Clin Microbiol Rev       Date:  1993-07       Impact factor: 26.132

6.  Identification of astrovirus serotypes from children treated at the Hospitals for Sick Children, London 1981-93.

Authors:  J Noel; D Cubitt
Journal:  Epidemiol Infect       Date:  1994-08       Impact factor: 2.451
  6 in total

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