| Literature DB >> 17186210 |
M D Adjei1, J Deck, T M Heinze, J P Freeman, A J Williams, J B Sutherland.
Abstract
Mycobacterium sp. 7E1B1W and seven other mycobacterial strains known to degrade hydrocarbons were investigated to determine their ability to metabolize the piperazine ring, a substructure found in many drugs. Cultures were grown at 30 degrees C in tryptic soy broth and dosed with 3.1 mM N-phenylpiperazine hydrochloride; samples were removed at intervals and extracted with ethyl acetate. Two metabolites were purified from each of the extracts by high-performance liquid chromatography; they were identified by mass spectrometry and (1)H nuclear magnetic resonance spectroscopy as N-(2-anilinoethyl)acetamide and N-acetyl-N'-phenylpiperazine. The results show that mycobacteria have the ability to acetylate piperazine rings and cleave carbon-nitrogen bonds.Entities:
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Year: 2006 PMID： 17186210 DOI： 10.1007/s10295-006-0189-x
Source DB: PubMed Journal: J Ind Microbiol Biotechnol ISSN： 1367-5435 Impact factor: 3.346