| Literature DB >> 17184935 |
Haiqiang Ning1, Zhongxiang Niu, Ruisong Yu, Peihua Zhang, Shijuan Dong, Zhen Li.
Abstract
Strains of hepatitis E virus (HEV) genotypes 1 and 4 have been detected on the Chinese mainland although there have been no previous reports of zoonotic genotype 3 HEV. In the present study, 65 swine fecal specimens were collected from five pig farms located in different Shanghai suburbs. RT-PCR and nested PCR were undertaken using partial nucleotide sequences of Open Reading Frame 2 (ORF2) of HEV to detect HEV RNA. Genetic analysis was based on alignments of an amplified 150-nt ORF2 sequence. RT-PCR revealed 15 HEV positive samples among 65-pig fecal specimens examined. Phylogenetic analysis of the amplified sequences indicated seven HEV strains belonged to genotype 3 and eight strains to genotype 4. This is the first time that genotype 3 hepatitis E virus has been identified on the Chinese mainland.Entities:
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Year: 2006 PMID： 17184935 DOI： 10.1016/j.vetmic.2006.11.006
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293