Min Hu1, Chang-Qin Hu. 1. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
Abstract
AIM: To identify the degradation compounds of cefathiamidine by a liquid chromatography-tandam mass spectrometry (LC-MS). METHODS: According to the accelerated storage condition, two main degradation compounds of cefathiamindine were its hydrolytic products. Cefathiamidine was separated on a C18 column, with 1% acetate solution-acetonitrile (85 : 15) as mobile phase. The mass spectra and MS/MS spectra were obtained with set at the positive ion mode. Combined the UV spectra and the chromatography behavior, the structures of two degradation compounds were identified. RESULTS: The method can be used for the separation of the degradation compounds of cefathiamindine. The two major degradation compounds were desacetylcefathiamidine and cefathiamidine lactone. CONCLUSION: The method is rapid, sensitive and specific, and it is suitable for the identification of the degradation compounds of cephalosporin.
AIM: To identify the degradation compounds of cefathiamidine by a liquid chromatography-tandam mass spectrometry (LC-MS). METHODS: According to the accelerated storage condition, two main degradation compounds of cefathiamindine were its hydrolytic products. Cefathiamidine was separated on a C18 column, with 1% acetate solution-acetonitrile (85 : 15) as mobile phase. The mass spectra and MS/MS spectra were obtained with set at the positive ion mode. Combined the UV spectra and the chromatography behavior, the structures of two degradation compounds were identified. RESULTS: The method can be used for the separation of the degradation compounds of cefathiamindine. The two major degradation compounds were desacetylcefathiamidine and cefathiamidine lactone. CONCLUSION: The method is rapid, sensitive and specific, and it is suitable for the identification of the degradation compounds of cephalosporin.