Literature DB >> 17183166

Purification, crystallization and preliminary crystallographic characterization of the caspase-recruitment domain of human Nod1.

Thiagarajan Srimathi1, Sheila L Robbins, Rachel L Dubas, Jang Hoon Seo, Young Chul Park.   

Abstract

The caspase-recruitment domain (CARD) is known to play an important role in apoptosis and inflammation as an essential protein-protein interaction domain. The CARD of the cytosolic pathogen receptor Nod1 was overexpressed in Escherichia coli and purified by affinity chromatography and gel filtration. The purified CARD was crystallized at 277 K using the microseeding method. X-ray diffraction data were collected to 1.9 A resolution. The crystals belong to space group P3(1) or P3(2), with unit-cell parameters a = b = 79.1, c = 80.9 A. Preliminary analysis indicates that there is one dimeric CARD molecule in the asymmetric unit.

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Year:  2006        PMID: 17183166      PMCID: PMC2330101          DOI: 10.1107/S1744309106051955

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN: 1744-3091


  12 in total

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