| Literature DB >> 17178102 |
Kaya Yakata1, Yoko Hiroaki, Kenichi Ishibashi, Eisei Sohara, Sei Sasaki, Kaoru Mitsuoka, Yoshinori Fujiyoshi.
Abstract
Recently, two novel mammalian aquaporins (AQPs), AQPs 11 and 12, have been identified and classified as members of a new AQP subfamily, the "subcellular AQPs". In members of this subfamily one of the two asparagine-proline-alanine (NPA) motifs, which play a crucial role in selective water conduction, are not completely conserved. Mouse AQP11 (mAQP11) was expressed in Sf9 cells and purified using the detergent Fos-choline 10. The protein was reconstituted into liposomes, which were used for water conduction studies with a stopped-flow device. Single water permeability (pf) of AQP11 was measured to be 1.72+/-0.03x10(-13) cm(3)/s, suggesting that other members of the subfamily with incompletely conserved NPA motifs may also function as water channels.Entities:
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Year: 2006 PMID： 17178102 DOI： 10.1016/j.bbamem.2006.11.005
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002