Literature DB >> 17177484

Equal performance of TaqMan, MGB, molecular beacon, and SYBR green-based detection assays in detection and quantification of roundup ready soybean.

Charlotte Bøydler Andersen1, Arne Holst-Jensen, Knut Gunnar Berdal, Tage Thorstensen, Torstein Tengs.   

Abstract

We have tested and compared the performance of 12 different assays representing four different real-time polymerase chain reaction (PCR) chemistries in the context of genetically modified organism detection. Several different molecular beacon, SYBR Green, TaqMan, and MGB assays were designed for the event specific detection and quantification of the 3' integration junction of GTS 40-3-2 (Roundup Ready) soybean. Sensitivity as well as robustness in the presence of background DNA were tested. None of the PCR-based approaches appeared to be significantly better than any of the other, but the molecular beacon assays had the lowest efficiency and also seemed more sensitive to changes in experimental setup.

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Year:  2006        PMID： 17177484     DOI： 10.1021/jf061987c

Source DB:  PubMed          Journal:  J Agric Food Chem        ISSN： 0021-8561            Impact factor:   5.279


  4 in total

1.  Real-time PCR quantification of the plant growth promoting bacteria Herbaspirillum seropedicae strain SmR1 in maize roots.

Authors:  Tomás Pellizzaro Pereira; Fernanda Plucani do Amaral; Pamela Dall'Asta; Fábio Cristiano Angonesi Brod; Ana Carolina Maisonnave Arisi
Journal:  Mol Biotechnol       Date:  2014-07       Impact factor: 2.695

2.  A specific endogenous reference for genetically modified common bean (Phaseolus vulgaris L.) DNA quantification by real-time PCR targeting lectin gene.

Authors:  Gustavo L Venturelli; Fábio C A Brod; Gabriela B Rossi; Naíra F Zimmermann; Jaison P Oliveira; Josias C Faria; Ana C M Arisi
Journal:  Mol Biotechnol       Date:  2014-11       Impact factor: 2.695

3.  Identification of GCC-box and TCC-box motifs in the promoters of differentially expressed genes in rice (Oryza sativa L.): Experimental and computational approaches.

Authors:  Gopal Kumar Prajapati; Bharati Pandey; Awdhesh Kumar Mishra; Kwang-Hyun Baek; Dev Mani Pandey
Journal:  PLoS One       Date:  2019-04-26       Impact factor: 3.240

4.  Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms.

Authors:  Meti Buh Gasparic; Katarina Cankar; Jana Zel; Kristina Gruden
Journal:  BMC Biotechnol       Date:  2008-03-06       Impact factor: 2.563
  4 in total

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