Literature DB >> 1717600

The GLP large scale preparation of immunotoxins containing deglycosylated ricin A chain and a hindered disulfide bond.

V Ghetie1, P Thorpe, M A Ghetie, P Knowles, J W Uhr, E S Vitetta.   

Abstract

The large scale preparation of two second generation immunotoxins containing murine monoclonal antibodies and deglycosylated ricin A chain is described. The procedure for the preparation of immunotoxins consists of the derivatization of antibody with SMPT and reduction of dgA with DTT followed by their reaction to establish a hindered interchain disulfide bond. The purification of the immunotoxin includes affinity chromatography on Blue-Sepharose to remove the free antibody and gel filtration on Sephacryl S-200HR to remove any high molecular weight material and free dgA. The two immunotoxins were prepared by GLP procedures and tested for yield, composition, purity, sterility and biological activity.

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Year:  1991        PMID： 1717600     DOI： 10.1016/0022-1759(91)90110-2

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  13 in total

1.  An anti-CD45RO immunotoxin kills HIV-latently infected cells from individuals on HAART with little effect on CD8 memory.

Authors:  J Saavedra-Lozano; Y Cao; J Callison; R Sarode; D Sodora; J Edgar; J Hatfield; L Picker; D Peterson; O Ramilo; E S Vitetta
Journal:  Proc Natl Acad Sci U S A       Date:  2004-02-24       Impact factor: 11.205

2.  Homodimerization of tumor-reactive monoclonal antibodies markedly increases their ability to induce growth arrest or apoptosis of tumor cells.

Authors:  M A Ghetie; E M Podar; A Ilgen; B E Gordon; J W Uhr; E S Vitetta
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-08       Impact factor: 11.205

Review 3.  Immunotoxins for the treatment of B-cell lymphomas.

Authors:  M A Ghetie; V Ghetie; E S Vitetta
Journal:  Mol Med       Date:  1997-07       Impact factor: 6.354

4.  Long-term outcome after haploidentical stem cell transplant and infusion of T cells expressing the inducible caspase 9 safety transgene.

Authors:  Xiaoou Zhou; Antonio Di Stasi; Siok-Keen Tey; Robert A Krance; Caridad Martinez; Kathryn S Leung; April G Durett; Meng-Fen Wu; Hao Liu; Ann M Leen; Barbara Savoldo; Yu-Feng Lin; Bambi J Grilley; Adrian P Gee; David M Spencer; Cliona M Rooney; Helen E Heslop; Malcolm K Brenner; Gianpietro Dotti
Journal:  Blood       Date:  2014-04-21       Impact factor: 22.113

5.  Identification of Human Anti-HIV gp160 Monoclonal Antibodies That Make Effective Immunotoxins.

Authors:  Seth H Pincus; Kejing Song; Grace A Maresh; Dean H Hamer; Dimiter S Dimitrov; Weizao Chen; Mei-Yun Zhang; Victor F Ghetie; Po-Ying Chan-Hui; James E Robinson; Ellen S Vitetta
Journal:  J Virol       Date:  2017-01-18       Impact factor: 5.103

6.  Design and In Vivo Characterization of Immunoconjugates Targeting HIV gp160.

Authors:  Seth H Pincus; Kejing Song; Grace A Maresh; Anderson Frank; David Worthylake; Hye-Kyung Chung; Patricia Polacino; Dean H Hamer; Cody P Coyne; Michael G Rosenblum; John W Marks; Gang Chen; Deborah Weiss; Victor Ghetie; Ellen S Vitetta; James E Robinson; Shiu-Lok Hu
Journal:  J Virol       Date:  2017-01-18       Impact factor: 5.103

7.  Targeted inhibition of CD133+ cells in oral cancer cell lines.

Authors:  M Damek-Poprawa; A Volgina; J Korostoff; T P Sollecito; M S Brose; B W O'Malley; S O Akintoye; J M DiRienzo
Journal:  J Dent Res       Date:  2011-01-10       Impact factor: 6.116

8.  Combined use of an immunotoxin and cyclosporine to prevent both activated and quiescent peripheral blood T cells from producing type 1 human immunodeficiency virus.

Authors:  K D Bell; O Ramilo; E S Vitetta
Journal:  Proc Natl Acad Sci U S A       Date:  1993-02-15       Impact factor: 11.205

9.  Inducible caspase 9 suicide gene to improve the safety of allodepleted T cells after haploidentical stem cell transplantation.

Authors:  Siok-Keen Tey; Gianpietro Dotti; Cliona M Rooney; Helen E Heslop; Malcolm K Brenner
Journal:  Biol Blood Marrow Transplant       Date:  2007-05-29       Impact factor: 5.742

10.  Adoptive immunotherapy with allodepleted donor T-cells improves immune reconstitution after haploidentical stem cell transplantation.

Authors:  Persis J Amrolia; Giada Muccioli-Casadei; Helen Huls; Stuart Adams; April Durett; Adrian Gee; Eric Yvon; Heidi Weiss; Mark Cobbold; H Bobby Gaspar; Cliona Rooney; Ingrid Kuehnle; Victor Ghetie; John Schindler; Robert Krance; Helen E Heslop; Paul Veys; Ellen Vitetta; Malcolm K Brenner
Journal:  Blood       Date:  2006-06-01       Impact factor: 22.113
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