| Literature DB >> 17174895 |
Svetlana Petruk1, Yurii Sedkov, Kristen M Riley, Jacob Hodgson, Francois Schweisguth, Susumu Hirose, James B Jaynes, Hugh W Brock, Alexander Mazo.
Abstract
Much of the genome is transcribed into long noncoding RNAs (ncRNAs). Previous data suggested that bithoraxoid (bxd) ncRNAs of the Drosophila bithorax complex (BX-C) prevent silencing of Ultrabithorax (Ubx) and recruit activating proteins of the trithorax group (trxG) to their maintenance elements (MEs). We found that, surprisingly, Ubx and several bxd ncRNAs are expressed in nonoverlapping patterns in both embryos and imaginal discs, suggesting that transcription of these ncRNAs is associated with repression, not activation, of Ubx. Our data rule out siRNA or miRNA-based mechanisms for repression by bxd ncRNAs. Rather, ncRNA transcription itself, acting in cis, represses Ubx. The Trithorax complex TAC1 binds the Ubx coding region in nuclei expressing Ubx, and the bxd region in nuclei not expressing Ubx. We propose that TAC1 promotes the mosaic pattern of Ubx expression by facilitating transcriptional elongation of bxd ncRNAs, which represses Ubx transcription.Entities:
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Year: 2006 PMID： 17174895 PMCID： PMC1866366 DOI： 10.1016/j.cell.2006.10.039
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582