BACKGROUND: Apolipoprotein A-I (apoA-I) is the major lipoprotein component of high-density lipoprotein(HDL), and plays an important role in reverse cholesterol transport. Its function is known to be influenced by oxidation. METHODS: Using H2O2-or chloramine T-oxidized apoA-I as antigen, we prepared 2 kinds of monoclonal antibodies, and established an ELISA system for the measurement of oxidized apoA-I. RESULTS: The 2 monoclonal antibodies obtained, 7D3 and 98A7, exhibited different reactivity characteristics. The serum level of oxidized apoA-I was higher in patients with either inflammatory disease or diabetes than in healthy individuals, and suggested a diversity of oxidized apoA-I. CONCLUSION: The 2 monoclonal antibodies are useful for the determination of oxidized apoA-I and study of diverse oxidized HDLs.
BACKGROUND:Apolipoprotein A-I (apoA-I) is the major lipoprotein component of high-density lipoprotein(HDL), and plays an important role in reverse cholesterol transport. Its function is known to be influenced by oxidation. METHODS: Using H2O2-or chloramine T-oxidized apoA-I as antigen, we prepared 2 kinds of monoclonal antibodies, and established an ELISA system for the measurement of oxidized apoA-I. RESULTS: The 2 monoclonal antibodies obtained, 7D3 and 98A7, exhibited different reactivity characteristics. The serum level of oxidized apoA-I was higher in patients with either inflammatory disease or diabetes than in healthy individuals, and suggested a diversity of oxidized apoA-I. CONCLUSION: The 2 monoclonal antibodies are useful for the determination of oxidized apoA-I and study of diverse oxidized HDLs.
Authors: Ji Ye Ahn; Il Yong Kim; Sae Jin Oh; Hye Sook Hwang; Sun Shin Yi; Yo Na Kim; Jae Hoon Shin; Yeo Sung Yoon; Je Kyung Seong Journal: Lab Anim Res Date: 2014-06-23