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Literature DB >> 1717409

A histochemical method for the demonstration of unsaturated lipids.

Abstract

A method has been developed for the histochemical demonstration of unsaturated lipids in light microscopy. It is a peracetic acid-thiocarbohydrazide-silver protein sequence followed by a physical development procedure. In the present study on paraffin and cryostat sections of liver, brain and ovary, unsaturated lipids were visualized as distinct reaction products coloured various shades of brown and black. The reaction products are easier to see and the method is more efficient than the peracetic acid-Schiff method.

Entities: Chemical

Mesh：

Substances：

Year: 1991 PMID： 1717409 DOI： 10.1007/bf01047113

Source DB: PubMed Journal: Histochem J ISSN： 0018-2214

13 in total

1. An efficient histochemical method for deoxyribonucleic acids using a silver enhancement procedure.

Authors: A Tsukise; O Fujimori; K Yamada
Journal: Histochem J Date: 1990-08

2. Applications of immunocolloids in light microscopy. IV. Use of photochemical silver staining in a simple and efficient double-staining technique.

Authors: C Manigley; J Roth
Journal: J Histochem Cytochem Date: 1985-12 Impact factor: 2.479

8. Lectin binding sites of glycoproteins as revealed by lectin-gold-silver and lectin-peroxidase-diaminobenzidine methods.

Authors: O Fujimori; H M Sobue; K Yamada
Journal: Histochem J Date: 1988-11

9. Histochemical demonstration of zinc in rat epididymis using a sulphide-silver method.

Authors: O Fujimori; A Tsukise; K Yamada
Journal: Histochemistry Date: 1988

10. Enhancement of the periodic acid--Schiff (PAS) and periodic acid--thiocarbohydrazide--silver proteinate (PA-TCH-SP) reaction in LR white sections.

Authors: W F Neiss
Journal: Histochemistry Date: 1988

3 in total

A histochemical method for the demonstration of unsaturated lipids.

1. An efficient histochemical method for deoxyribonucleic acids using a silver enhancement procedure.

2. Applications of immunocolloids in light microscopy. IV. Use of photochemical silver staining in a simple and efficient double-staining technique.

3. A method for histochemical demonstration of protein-bound amino groups by light microscopy.

4. Morphochemical analysis of mucous cells in the skin and slime glands of hagfishes.

5. Protein A gold-silver staining method for light microscopic immunohistochemistry.

6. A sensitive method for the histochemical demonstration of vicinal diols of carbohydrates.

7. The potential of the immunogold-silver staining method for paraffin sections.

8. Lectin binding sites of glycoproteins as revealed by lectin-gold-silver and lectin-peroxidase-diaminobenzidine methods.

9. Histochemical demonstration of zinc in rat epididymis using a sulphide-silver method.

10. Enhancement of the periodic acid--Schiff (PAS) and periodic acid--thiocarbohydrazide--silver proteinate (PA-TCH-SP) reaction in LR white sections.

1. A double protein A-gold-silver staining method for tissue antigens in light microscopy.

Review 2. Histochemistry of carbohydrates as performed by physical development procedures.

3. Light-microscopic detection of acidic glycoconjugates with sensitized diamine procedures.