Literature DB >> 17172730

Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells.

Holger Erfle1, Rainer Pepperkok.   

Abstract

In this chapter, we provide a protocol for the production of transfected cell arrays in living mammalian cells on noncoated chambered coverglass for the systematic functional analyses of human genes by high-content screening microscopy. This method should facilitate drug target validation by small-interfering RNAs.

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Year:  2007        PMID： 17172730     DOI： 10.1385/1-59745-165-7:155

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

Review 1.  Automated microscopy for high-content RNAi screening.

Authors:  Christian Conrad; Daniel W Gerlich
Journal:  J Cell Biol       Date:  2010-02-22       Impact factor: 10.539

2.  Visual genome-wide RNAi screening to identify human host factors required for Trypanosoma cruzi infection.

Authors:  Auguste Genovesio; Miriam A Giardini; Yong-Jun Kwon; Fernando de Macedo Dossin; Seo Yeon Choi; Nam Youl Kim; Hi Chul Kim; Sung Yong Jung; Sergio Schenkman; Igor C Almeida; Neil Emans; Lucio H Freitas-Junior
Journal:  PLoS One       Date:  2011-05-20       Impact factor: 3.240

3.  Normalizing for individual cell population context in the analysis of high-content cellular screens.

Authors:  Bettina Knapp; Ilka Rebhan; Anil Kumar; Petr Matula; Narsis A Kiani; Marco Binder; Holger Erfle; Karl Rohr; Roland Eils; Ralf Bartenschlager; Lars Kaderali
Journal:  BMC Bioinformatics       Date:  2011-12-20       Impact factor: 3.169

Review 4.  Living Cell Microarrays: An Overview of Concepts.

Authors:  Rebecca Jonczyk; Tracy Kurth; Antonina Lavrentieva; Johanna-Gabriela Walter; Thomas Scheper; Frank Stahl
Journal:  Microarrays (Basel)       Date:  2016-05-26
  4 in total

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