| Literature DB >> 17172680 |
Mariana Eça Guimarães de Araújo1, Lukas Alfons Huber.
Abstract
The successful combination of highly sensitive mass spectrometry and pre-fractionation techniques has provided a powerful tool to detect dynamic changes in low abundant regulatory proteins at the organelle level. Subcellular fractionation, being flexible, adjustable (both in cell and tissues), and allowing the analysis of proteins in their physiologic/intracellular context, has become the most commonly used preparative/enrichment method. This chapter introduces state-of-the-art subcellular fractionation protocols and briefly discuss their suitability, advantages, and limitations.Mesh：
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Year: 2007 PMID： 17172680 DOI： 10.1385/1-59745-214-9:73
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745