Literature DB >> 17166969

Use of pulsed-field gel electrophoresis of conserved XbaI fragments for identification of swine Salmonella serotypes.

Stephen B Gaul1, Stephanie Wedel, Matthew M Erdman, D L Harris, Isabel Turney Harris, Kathleen E Ferris, Lorraine Hoffman.   

Abstract

Swine Salmonella isolates (n=674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with XbaI. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using XbaI restriction provided a possible alternative method for screening and identifying swine Salmonella serotypes.

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Year:  2006        PMID： 17166969      PMCID： PMC1829035          DOI： 10.1128/JCM.00962-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

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Review 4.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.

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5.  Recombinational basis of serovar diversity in Salmonella enterica.

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6.  Molecular typing of Salmonella serotypes prevalent in animals in England: assessment of methodology.

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  11 in total

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6.  Characterization of 13 multi-drug resistant Salmonella serovars from different broiler chickens associated with those of human isolates.

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