Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Establishment of an alpha-fetoprotein-producing gastric cancer cell line in serum-free media.

Literature DB >> 1716623

Establishment of an alpha-fetoprotein-producing gastric cancer cell line in serum-free media.

M Terashima¹, K Ikeda, C Maesawa, H Kawamura, Y Niitsu, M Satoh, K Saito.

Abstract

A new cell line, designated ISt-1, was established in serum-free medium from a 63-year-old gastric cancer patient with an extremely high serum level of alpha-fetoprotein (AFP). ISt-1 exhibited a typical morphology of epithelial cells in culture. The population doubling time was 31 hours. Production of AFP was demonstrated by immunohistochemical study and high levels of AFP were detected in the conditioned medium of ISt-1 cells. ISt-1 is considered to be an AFP-producing gastric cancer cell line and will provide a useful tool for the study of production and secretion of AFP from cells.

Entities: CellLine Disease Gene Species

Mesh：

Substances：

Year: 1991 PMID： 1716623 PMCID： PMC5918585 DOI： 10.1111/j.1349-7006.1991.tb01915.x

Source DB: PubMed Journal: Jpn J Cancer Res ISSN： 0910-5050

11 in total

1. Synthesis of -fetoprotein by liver, yolk sac, and gastrointestinal tract of the human conceptus.

Authors: D Gitlin; A Perricelli; G M Gitlin
Journal: Cancer Res Date: 1972-05 Impact factor: 12.701

2. Production of embryonal serum alpha-globulin by hepatomas: review of experimental and clinical data.

Authors: G I Abelev
Journal: Cancer Res Date: 1968-07 Impact factor: 12.701

3. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

Authors: S M Hsu; L Raine; H Fanger
Journal: J Histochem Cytochem Date: 1981-04 Impact factor: 2.479

4. Characteristics of cell lines established from human gastric carcinoma.

Authors: J G Park; H Frucht; R V LaRocca; D P Bliss; Y Kurita; T R Chen; J G Henslee; J B Trepel; R T Jensen; B E Johnson
Journal: Cancer Res Date: 1990-05-01 Impact factor: 12.701

5. Production of alpha-fetoprotein, normal serum proteins, and human chorionic gonadotropin in stomach cancer: histologic and immunohistochemical analyses of 35 cases.

Authors: T Kodama; T Kameya; T Hirota; Y Shimosato; H Ohkura; T Mukojima; H Kitaoka
Journal: Cancer Date: 1981-10-01 Impact factor: 6.860

6. Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.

Authors: H Nakabayashi; K Taketa; K Miyano; T Yamane; J Sato
Journal: Cancer Res Date: 1982-09 Impact factor: 12.701

7. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.

Authors: B B Knowles; C C Howe; D P Aden
Journal: Science Date: 1980-07-25 Impact factor: 47.728

8. Serum alpha-fetoprotein in patients with neoplasms of the gastrointestinal tract.

Authors: K R McIntire; T A Waldmann; C G Moertel; V L Go
Journal: Cancer Res Date: 1975-04 Impact factor: 12.701

9. Establishment of an AFP-producing serum-independent culture cell line of human liver cancer and changes in protein synthesis associated with the absence of serum (First Part).

Authors: T Nohara
Journal: Gastroenterol Jpn Date: 1987-12

4. Establishment of two new scirrhous gastric cancer cell lines: analysis of factors associated with disseminated metastasis.

Authors: M Yashiro; Y S Chung; S Nishimura; T Inoue; M Sowa
Journal: Br J Cancer Date: 1995-11 Impact factor: 7.640

4 in total