Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Reversible effects of chaotropic agents on the proton permeability of Escherichia coli membrane vesicles.

Literature DB >> 171661

Reversible effects of chaotropic agents on the proton permeability of Escherichia coli membrane vesicles.

Abstract

Extraction of E. coli ML 308-225 membrane vesicles with chaotropic agents causes the vesicles to become specifically permeable to protons. As a result, the vesicles no longer generate a membrane potential, interior negative, and they do not catalyze respiration-dependent lactose or proline transport. Treatment of the extracted vesicles with various carbodiimides decreases the permeability of the vesicle membrane to protons, causing them to regain their ability to generate a membrane potential. By this means, active transport is completely reactivated. Exposure of the vesicles to carbodiimides prior to extraction with chaotropic agents makes transport activity impervious to the effects of the chaotropes.

Entities: Chemical Species

Mesh：

Substances：

Year: 1975 PMID： 171661 PMCID： PMC432998 DOI： 10.1073/pnas.72.9.3387

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

38 in total

1. Energy-dependent binding of dansylgalactosides to the beta-galactoside carrier protein.

Authors: S Schuldiner; G K Kerwar; H R Kaback; R Weil
Journal: J Biol Chem Date: 1975-02-25 Impact factor: 5.157

2. RESTORATION OF OXIDATIVE PHOSPHORYLATION IN NON-PHOSPHORYLATING SUBMITOCHONDRIAL PARTICLES BY OLIGOMYCIN.

Authors: C P LEE; L ERNSTER
Journal: Biochem Biophys Res Commun Date: 1965-02-17 Impact factor: 3.575

3. Proton-translocation phosphorylation in mitochondria, chloroplasts and bacteria: natural fuel cells and solar cells.

Authors: P Mitchell
Journal: Fed Proc Date: 1967-09

4. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.

Authors: G F Ames
Journal: J Biol Chem Date: 1974-01-25 Impact factor: 5.157

Review 5. Conservation and transformation of energy by bacterial membranes.

Authors: F M Harold
Journal: Bacteriol Rev Date: 1972-06

6. Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles.

Authors: S A Short; H R Kaback; L D Kohn
Journal: J Biol Chem Date: 1975-06-10 Impact factor: 5.157

7. New procedure for the isolation of membrane vesicles of Bacillus subtilis and an electron microscopy study of their ultrastructure.

Authors: W N Konings; A Bisschop; M Veenhuis; C A Vermeulen
Journal: J Bacteriol Date: 1973-12 Impact factor: 3.490

8. Energy conservation in membranes of mutants of Escherichia coli defective in oxidative phosphorylation.

Authors: F J Nieuwenhuis; B I Kanner; D L Gutnick; P W Postma; K van Dam
Journal: Biochim Biophys Acta Date: 1973-10-19

9. Accumulation of lipid-soluble ions and of rubidium as indicators of the electrical potential in membrane vesicles of Escherichia coli.

Authors: K Altendorf; H Hirata; F M Harold
Journal: J Biol Chem Date: 1975-02-25 Impact factor: 5.157

10. Photoinactivation of the beta-galactoside transport system in Escherichia coli membrane vesicles with 2-nitro-4-azidophenyl-1-thio-beta-D-galactopyranoside.

Authors: G Rudnick; H R Kaback; R Weil
Journal: J Biol Chem Date: 1975-02-25 Impact factor: 5.157

16 in total

1. Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation.

Authors: N Nelson; E Eytan; B E Notsani; H Sigrist; K Sigrist-Nelson; C Gitler
Journal: Proc Natl Acad Sci U S A Date: 1977-06 Impact factor: 11.205

Review 2. In vitro translocation of bacterial secretory proteins and energy requirements.

Authors: S Mizushima; H Tokuda
Journal: J Bioenerg Biomembr Date: 1990-06 Impact factor: 2.945

3. The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles.

Authors: S Ramos; S Schuldiner; H R Kaback
Journal: Proc Natl Acad Sci U S A Date: 1976-06 Impact factor: 11.205

Reversible effects of chaotropic agents on the proton permeability of Escherichia coli membrane vesicles.

1. Energy-dependent binding of dansylgalactosides to the beta-galactoside carrier protein.

2. RESTORATION OF OXIDATIVE PHOSPHORYLATION IN NON-PHOSPHORYLATING SUBMITOCHONDRIAL PARTICLES BY OLIGOMYCIN.

3. Proton-translocation phosphorylation in mitochondria, chloroplasts and bacteria: natural fuel cells and solar cells.

4. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.

Review 5. Conservation and transformation of energy by bacterial membranes.

6. Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles.

7. New procedure for the isolation of membrane vesicles of Bacillus subtilis and an electron microscopy study of their ultrastructure.

8. Energy conservation in membranes of mutants of Escherichia coli defective in oxidative phosphorylation.

9. Accumulation of lipid-soluble ions and of rubidium as indicators of the electrical potential in membrane vesicles of Escherichia coli.

10. Photoinactivation of the beta-galactoside transport system in Escherichia coli membrane vesicles with 2-nitro-4-azidophenyl-1-thio-beta-D-galactopyranoside.

1. Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation.

Review 2. In vitro translocation of bacterial secretory proteins and energy requirements.

3. The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles.

4. Transport-defective Escherichia coli ecf mutant permeable to protons and nucleotides.

Review 5. Transport of H+, K+, Na+ and Ca++ in Streptococcus.

Review 6. The lac carrier protein in Escherichia coli.

7. Electrochemical proton gradient in Micrococcus lysodeikticus cells and membrane vesicles.

8. Membrane permeability of fructose-1,6-diphosphate in lipid vesicles and endothelial cells.

9. Alteration of the Escherichia coli membrane by addition of bacteriophage T4 protein synthesized after infection.

10. Membrane potential and gentamicin uptake in Staphylococcus aureus.