Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Maximizing DNA loading on a range of gold nanoparticle sizes.

Literature DB >> 17165821

Maximizing DNA loading on a range of gold nanoparticle sizes.

Sarah J Hurst¹, Abigail K R Lytton-Jean, Chad A Mirkin.

Abstract

We have investigated the variables that influence DNA coverage on gold nanoparticles. The effects of salt concentration, spacer composition, nanoparticle size, and degree of sonication have been evaluated. Maximum loading was obtained by salt aging the nanoparticles to approximately 0.7 M NaCl in the presence of DNA containing a poly(ethylene glycol) spacer. In addition, DNA loading was substantially increased by sonicating the nanoparticles during the surface loading process. Last, nanoparticles up to 250 nm in diameter were found have approximately 2 orders of magnitude higher DNA loading than smaller (13-30 nm) nanoparticles, a consequence of their larger surface area. Stable large particles are attractive for a variety of biodiagnostic assays.

Entities: Chemical

Mesh：

Substances：

Year: 2006 PMID： 17165821 PMCID： PMC2525614 DOI： 10.1021/ac0613582

Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986

32 in total

1. Amplified detection of DNA and analysis of single-base mismatches by the catalyzed deposition of gold on Au-nanoparticles.

Authors: Y Weizmann; F Patolsky; I Willner
Journal: Analyst Date: 2001-09 Impact factor: 4.616

2. Thermal desorption behavior and binding properties of DNA bases and nucleosides on gold.

Authors: Linette M Demers; Mattias Ostblom; Hua Zhang; Nak-Han Jang; Bo Liedberg; Chad A Mirkin
Journal: J Am Chem Soc Date: 2002-09-25 Impact factor: 15.419

3. Multiplexed detection of protein cancer markers with biobarcoded nanoparticle probes.

Authors: Savka I Stoeva; Jae-Seung Lee; Jennifer E Smith; Steven T Rosen; Chad A Mirkin
Journal: J Am Chem Soc Date: 2006-07-05 Impact factor: 15.419

4. Nucleobase orientation and ordering in films of single-stranded DNA on gold.

Authors: Dmitri Y Petrovykh; V Pérez-Dieste; Aric Opdahl; Hiromi Kimura-Suda; J M Sullivan; Michael J Tarlov; F J Himpsel; Lloyd J Whitman
Journal: J Am Chem Soc Date: 2006-01-11 Impact factor: 15.419

5. Determining the conformation of thiolated poly(ethylene glycol) on Au nanoshells by surface-enhanced Raman scattering spectroscopic assay.

Authors: Carly S Levin; Sandra Whaley Bishnoi; Nathaniel K Grady; Naomi J Halas
Journal: Anal Chem Date: 2006-05-15 Impact factor: 6.986

6. A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles.

Authors: L M Demers; C A Mirkin; R C Mucic; R A Reynolds; R L Letsinger; R Elghanian; G Viswanadham
Journal: Anal Chem Date: 2000-11-15 Impact factor: 6.986

7. On the structure and desorption dynamics of DNA bases adsorbed on gold: a temperature-programmed study.

Authors: Mattias Ostblom; Bo Liedberg; Linette M Demers; Chad A Mirkin
Journal: J Phys Chem B Date: 2005-08-11 Impact factor: 2.991

8. Raman and FTIR spectroscopies of fluorescein in solutions.

Authors: L Wang; A Roitberg; C Meuse; A K Gaigalas
Journal: Spectrochim Acta A Mol Biomol Spectrosc Date: 2001-08 Impact factor: 4.098

9. Nanoparticle-based detection in cerebral spinal fluid of a soluble pathogenic biomarker for Alzheimer's disease.

Authors: Dimitra G Georganopoulou; Lei Chang; Jwa-Min Nam; C Shad Thaxton; Elliott J Mufson; William L Klein; Chad A Mirkin
Journal: Proc Natl Acad Sci U S A Date: 2005-02-04 Impact factor: 11.205

10. Magnetically assisted DNA assays: high selectivity using conjugated polymers for amplified fluorescent transduction.

Authors: Hui Xu; Haiping Wu; Fei Huang; Shiping Song; Wenxin Li; Yong Cao; Chunhai Fan
Journal: Nucleic Acids Res Date: 2005-05-19 Impact factor: 16.971

158 in total

1. Biocompatible infinite-coordination-polymer nanoparticle-nucleic-acid conjugates for antisense gene regulation.

Authors: Colin M Calabrese; Chad A Mirkin; Timothy J Merkel; William E Briley; Pratik S Randeria; Suguna P Narayan; Jessica L Rouge; David A Walker; Alexander W Scott
Journal: Angew Chem Int Ed Engl Date: 2014-11-13 Impact factor: 15.336

2. Polyvalent nucleic acid nanostructures.

Authors: Joshua I Cutler; Ke Zhang; Dan Zheng; Evelyn Auyeung; Andrew E Prigodich; Chad A Mirkin
Journal: J Am Chem Soc Date: 2011-06-01 Impact factor: 15.419

3. Tailoring DNA structure to increase target hybridization kinetics on surfaces.

Authors: Andrew E Prigodich; One-Sun Lee; Weston L Daniel; Dwight S Seferos; George C Schatz; Chad A Mirkin
Journal: J Am Chem Soc Date: 2010-08-11 Impact factor: 15.419

4. Transfer of molecular recognition information from DNA nanostructures to gold nanoparticles.

Authors: Thomas G W Edwardson; Kai Lin Lau; Danny Bousmail; Christopher J Serpell; Hanadi F Sleiman
Journal: Nat Chem Date: 2016-01-04 Impact factor: 24.427

5. High relaxivity Gd(III)-DNA gold nanostars: investigation of shape effects on proton relaxation.

Authors: Matthew W Rotz; Kayla S B Culver; Giacomo Parigi; Keith W MacRenaris; Claudio Luchinat; Teri W Odom; Thomas J Meade
Journal: ACS Nano Date: 2015-03-24 Impact factor: 15.881

6. Monitoring the Photocleaving Dynamics of Colloidal MicroRNA-Functionalized Gold Nanoparticles Using Second Harmonic Generation.

Authors: Raju R Kumal; Corey R Landry; Mohammad Abu-Laban; Daniel J Hayes; Louis H Haber
Journal: Langmuir Date: 2015-09-03 Impact factor: 3.882