| Literature DB >> 17165744 |
Abstract
The mechanism of retaining glycosyltransferases is still poorly understood and the subject of current debate. Both double displacement and front side single displacement (SNi) mechanisms have been proposed. A "chemical rescue methodology" is here applied to a retaining alpha3-galactosyltransferase. Azide as exogenous nucleophile rescues the activity of the inactive E317A mutant to give beta-d-galactosylazide. This result fits best with a double displacement mechanism in which Glu317 is the enzyme nucleophile involved in the formation of a glycosyl-enzyme intermediate.Entities:
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Year: 2006 PMID： 17165744 DOI： 10.1021/ja0659931
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419