Meili Tian1, Min Wang. 1. School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
Abstract
OBJECTIVE: To study the extraction, isolation and composition of Lycium barbarum polysaccharides (LBP). METHOD: LBP was extracted from L. barbarum with water, isolationed and purified by DEAE ion-exchange cellulose and gel chromatography, and their structural composition was studied by means of SDS-PAGE gel electrophoresis, GC, amino acid automatic analysis, etc. RESULT: Pure LBP has four water solubie polysaccharides, M W was 1.524 x 10(5). LBP was composed of 6 kinds of monosaccharides (Ara, Rha, Xyl, Man, Gal and Glc), galacturonic acid and 18 kinds of amino acids. CONCLUSION: LBP is a kind of complex polysaccharides consisting of acidic heteropolysaccharides and polypeptide or protein, and LBP has Glycan-O-Ser glycopeptide structures.
OBJECTIVE: To study the extraction, isolation and composition of Lycium barbarumpolysaccharides (LBP). METHOD: LBP was extracted from L. barbarum with water, isolationed and purified by DEAE ion-exchange cellulose and gel chromatography, and their structural composition was studied by means of SDS-PAGE gel electrophoresis, GC, amino acid automatic analysis, etc. RESULT: Pure LBP has four water solubie polysaccharides, M W was 1.524 x 10(5). LBP was composed of 6 kinds of monosaccharides (Ara, Rha, Xyl, Man, Gal and Glc), galacturonic acid and 18 kinds of amino acids. CONCLUSION: LBP is a kind of complex polysaccharides consisting of acidic heteropolysaccharides and polypeptide or protein, and LBP has Glycan-O-Ser glycopeptide structures.
Authors: Gary D Stoner; Li-Shu Wang; Claire Seguin; Claudio Rocha; Kristen Stoner; Steven Chiu; A Douglas Kinghorn Journal: Pharm Res Date: 2010-03-16 Impact factor: 4.200