| Literature DB >> 17165236 |
M J Buttner1, I M Fearnley, M J Bibb.
Abstract
The DNA sequence of a 1.77 kb region of the Streptomyces coelicolor chromosome containing the coding and regulatory regions of the extracellular agarase (dagA) gene was determined. The sequence predicts a primary translation product of 309 amino acids and Mr 35132. Comparison of the N-terminal sequence determined for the mature extracellular protein with that of the primary translation product deduced from the DNA sequence predicts the presence of a 30 amino acid signal peptide. Analysis of the transcription of the dagA gene using high resolution S1 mapping, in vitro transcription, dinucleotide-primed in vitro transcription and in vivo promoter probing identified four promoters, initiating transcription approximately 32, 77, 125 and 200 nucleotides upstream of the coding sequence.Entities:
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Year: 1987 PMID： 17165236 DOI： 10.1007/bf00329843
Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925