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Literature DB >> 17163711

Single-pair FRET characterization of DNA tweezers.

Barbara K Müller¹, Andreas Reuter, Friedrich C Simmel, Don C Lamb.

Abstract

The self-assembly properties of DNA make it an ideal choice for the construction of nanomachines such as DNA tweezers. Because nanomachines function as individual units, they need to be studied on the single-molecule level. From single-pair FRET investigations, we show that "open" tweezers exist in a single conformation with minimal FRET efficiency, whereas upon addition of a "closing strand", three conformations are observed, which are averaged out in ensemble measurements.

Mesh：

Substances：
Oligonucleotides
DNA

Year: 2006 PMID： 17163711 DOI： 10.1021/nl0619406

Source DB: PubMed Journal: Nano Lett ISSN： 1530-6984 Impact factor: 11.189

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Cited

10 in total

1. Temperature-dependent FRET spectroscopy for the high-throughput analysis of self-assembled DNA nanostructures in real time.

Authors: Barbara Saccà; Rebecca Meyer; Christof M Niemeyer
Journal: Nat Protoc Date: 2009 Impact factor: 13.491

2. Timing molecular motion and production with a synthetic transcriptional clock.

Authors: Elisa Franco; Eike Friedrichs; Jongmin Kim; Ralf Jungmann; Richard Murray; Erik Winfree; Friedrich C Simmel
Journal: Proc Natl Acad Sci U S A Date: 2011-09-15 Impact factor: 11.205

Review 3. Biomedical detection via macro- and nano-sensors fabricated with metallic and semiconducting oxides.

Authors: Jong-In Hahm
Journal: J Biomed Nanotechnol Date: 2013-01 Impact factor: 4.099

4. Detecting intramolecular conformational dynamics of single molecules in short distance range with subnanometer sensitivity.

Authors: Ruobo Zhou; Simone Kunzelmann; Martin R Webb; Taekjip Ha
Journal: Nano Lett Date: 2011-10-27 Impact factor: 11.189

Single-pair FRET characterization of DNA tweezers.

1. Temperature-dependent FRET spectroscopy for the high-throughput analysis of self-assembled DNA nanostructures in real time.

2. Timing molecular motion and production with a synthetic transcriptional clock.

Review 3. Biomedical detection via macro- and nano-sensors fabricated with metallic and semiconducting oxides.

4. Detecting intramolecular conformational dynamics of single molecules in short distance range with subnanometer sensitivity.

5. Reversible light switch for macrocycle mobility in a DNA rotaxane.

6. Optical Voltage Sensing Using DNA Origami.

7. Different fluorophore labeling strategies and designs affect millisecond kinetics of DNA hairpins.

Review 8. Through the Eyes of Creators: Observing Artificial Molecular Motors.

9. Asymmetric patterning drives the folding of a tripodal DNA nanotweezer.

Review 10. Single-Molecule FRET: A Tool to Characterize DNA Nanostructures.