Literature DB >> 17163643

Seeing is believing: real-time cellular activity assay for phospholipase A2.

Wonhwa Cho1.   

Abstract

Quantitative real-time in situ activity assays are necessary for determining the physiological function and regulation of enzymes. A paper in this issue reports the synthesis of a series of new fluorogenic phospholipids that allow fast real-time measurements of cellular activity and head group selectivity of an important family of enzyme, phospholipases.

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Year:  2006        PMID: 17163643     DOI: 10.1021/cb600079t

Source DB:  PubMed          Journal:  ACS Chem Biol        ISSN: 1554-8929            Impact factor:   5.100


  2 in total

1.  Synthesis of mixed-chain phosphatidylcholines including coumarin fluorophores for FRET-based kinetic studies of phospholipase A(2) enzymes.

Authors:  Manlin Wang; Susmitha Pinnamaraju; Radha Ranganathan; Joseph Hajdu
Journal:  Chem Phys Lipids       Date:  2013-05-30       Impact factor: 3.329

2.  Activity-based targeting of secretory phospholipase A2 enzymes: A fatty-acid-binding-protein assisted approach.

Authors:  Amir Keshavarz; Ligia Zelaya; Jasmeet Singh; Radha Ranganathan; Joseph Hajdu
Journal:  Chem Phys Lipids       Date:  2016-11-25       Impact factor: 3.329

  2 in total

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