| Literature DB >> 17163436 |
Daniele Belluoccio1, Richard Wilson, David J Thornton, Tristan P Wallis, Jeffrey J Gorman, John F Bateman.
Abstract
Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.Entities:
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Year: 2006 PMID: 17163436 DOI: 10.1002/pmic.200600191
Source DB: PubMed Journal: Proteomics ISSN: 1615-9853 Impact factor: 3.984