Literature DB >> 17161700

Purification of hematopoietic stem cells using the side population.

K K Lin1, Margaret A Goodell.   

Abstract

Hematopoietic stem cells (HSCs) primarily reside in bone marrow, are defined by their ability to maintain blood homeostasis, and replenish themselves through self-renewal. Although HSC purification schemes vary from laboratory to laboratory, the resulting cell populations are similar, if not the same. This chapter will discuss different enrichment methods for HSCs and provide a detailed protocol for staining HSC with Hoechst 33342 for the side population (SP).

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Year:  2006        PMID： 17161700     DOI： 10.1016/S0076-6879(06)20011-9

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


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