PURPOSE: The feasibility of culturing murine adrenal cortical cells before transplantation was investigated in this study. METHOD: Primary murine adrenal cortical cells were maintained in either fetal bovine and horse sera-containing media or serum-free media. Real-time polymerase chain reaction was used to quantify the levels of adrenal cortical gene expression in the cultured cells. RESULTS: The use of sera-containing media led to the growth of many cells in the culture, but the expression of Sf-1, Dax-1, and Cyp11b1 in such cultures declined rapidly. In contrast, there was no significant cell growth in the serum-free culture medium. Culturing murine adrenal cortical cells in the serum-free medium resulted in higher levels of Sf-1, Dax-1, and Cyp11b1 gene expression. In the serum-free medium, adrenal cortical cells also responded to adrenocorticotropic hormone by increasing the expression of Cyp11b1 and suppressing the expression of Dax-1 in a dose-dependent manner. The addition of basic fibroblast growth factor to the serum-free medium maintained the expression of Sf-1, Dax-1, and Cyp11b1 for 4 weeks. CONCLUSION: Adrenal cortical cells isolated from adult mice were successfully maintained in a serum-free culture medium with basic fibroblast growth factor. This culture system may be suitable for further manipulation of adrenal cortical cells in vitro before transplantation.
PURPOSE: The feasibility of culturing murine adrenal cortical cells before transplantation was investigated in this study. METHOD: Primary murine adrenal cortical cells were maintained in either fetal bovine and horse sera-containing media or serum-free media. Real-time polymerase chain reaction was used to quantify the levels of adrenal cortical gene expression in the cultured cells. RESULTS: The use of sera-containing media led to the growth of many cells in the culture, but the expression of Sf-1, Dax-1, and Cyp11b1 in such cultures declined rapidly. In contrast, there was no significant cell growth in the serum-free culture medium. Culturing murine adrenal cortical cells in the serum-free medium resulted in higher levels of Sf-1, Dax-1, and Cyp11b1 gene expression. In the serum-free medium, adrenal cortical cells also responded to adrenocorticotropic hormone by increasing the expression of Cyp11b1 and suppressing the expression of Dax-1 in a dose-dependent manner. The addition of basic fibroblast growth factor to the serum-free medium maintained the expression of Sf-1, Dax-1, and Cyp11b1 for 4 weeks. CONCLUSION: Adrenal cortical cells isolated from adult mice were successfully maintained in a serum-free culture medium with basic fibroblast growth factor. This culture system may be suitable for further manipulation of adrenal cortical cells in vitro before transplantation.
Authors: Manikandan Subramanian; Crystal D Hayes; Joseph J Thome; Edward Thorp; Glenn K Matsushima; Joachim Herz; Donna L Farber; Kang Liu; Madepalli Lakshmana; Ira Tabas Journal: J Clin Invest Date: 2014-02-10 Impact factor: 14.808
Authors: Soojung Claire Hur; Tatiana Z Brinckerhoff; Christopher M Walthers; James C Y Dunn; Dino Di Carlo Journal: PLoS One Date: 2012-10-04 Impact factor: 3.240
Authors: Juan Bai; Karthi Duraisamy; Sarah O K Mak; Ahmed Allam; Jamaan Ajarem; Zhang Li; Billy K C Chow Journal: PLoS One Date: 2019-09-03 Impact factor: 3.240