[Determination of Hypoderma bovis and Przhevalskiana silenus somatic antigens using SDS-PAGE.].

The aim of this study was to examine larval antigens obtained from the third instar larvae of Hypoderma bovis and Przhevalskiana silenus using the sodium dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method. SDS-PAGE separation was performed using 12% separation gel and 5% stacking gel. At the end of the SDS-PAGE examination, 11 and 19 different polypeptide bands were detected between 6-66 kDa and 6-100 kDa in the third instar larvae antigen of H. bovis and P. silenus, respectively.