| Literature DB >> 1715977 |
Abstract
Using T7 RNA polymerase and specific constructs derived from 5S rRNA and RNA I genes, we generated substrates for the RNA processing enzyme RNase E. Using these substrates we have shown that a 3.2 kb DNA fragment that complements the rne-3071 mutation can express RNase E activity. We also found that T7 RNA polymerase terminates within the 5S rRNA gene.Entities:
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Year: 1991 PMID: 1715977 DOI: 10.1007/bf00282446
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925