Literature DB >> 1715977

The rne gene is the structural gene for the processing endoribonuclease RNase E of Escherichia coli.

A K Chauhan1, D Apirion.   

Abstract

Using T7 RNA polymerase and specific constructs derived from 5S rRNA and RNA I genes, we generated substrates for the RNA processing enzyme RNase E. Using these substrates we have shown that a 3.2 kb DNA fragment that complements the rne-3071 mutation can express RNase E activity. We also found that T7 RNA polymerase terminates within the 5S rRNA gene.

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Year:  1991        PMID: 1715977     DOI: 10.1007/bf00282446

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN: 0026-8925


  21 in total

1.  Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E. coli.

Authors:  B K Ghora; D Apirion
Journal:  Cell       Date:  1978-11       Impact factor: 41.582

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Initiation, processing and termination of ribosomal RNA from a hybrid 5 S ribosomal RNA gene in a plasmid.

Authors:  J Szeberényi; D Apirion
Journal:  J Mol Biol       Date:  1983-08-15       Impact factor: 5.469

4.  New M13 vectors for cloning.

Authors:  J Messing
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

5.  Fusion of the tandem Escherichia coli rrnA promoters to a transcription termination signal from the end of rrnD.

Authors:  R M Elford; W M Holmes
Journal:  J Mol Biol       Date:  1983-08-15       Impact factor: 5.469

6.  The gene specifying RNase E (rne) and a gene affecting mRNA stability (ams) are the same gene.

Authors:  L Taraseviciene; A Miczak; D Apirion
Journal:  Mol Microbiol       Date:  1991-04       Impact factor: 3.501

7.  Multiple pathways for primary processing of ribosomal RNA in Escherichia coli.

Authors:  P Gegenheimer; N Watson; D Apirion
Journal:  J Biol Chem       Date:  1977-05-10       Impact factor: 5.157

8.  Inactivation of the ribonucleic acid-processing enzyme ribonuclease E blocks cell division.

Authors:  K Goldblum; D Apririon
Journal:  J Bacteriol       Date:  1981-04       Impact factor: 3.490

9.  A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

Authors:  S Tabor; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

10.  7 S RNA: a single site substrate for the RNA processing enzyme ribonuclease E of Escherichia coli.

Authors:  J Szeberényi; M K Roy; D Apirion
Journal:  Biochim Biophys Acta       Date:  1983-08-02
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  1 in total

1.  The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.

Authors:  K J McDowall; R G Hernandez; S Lin-Chao; S N Cohen
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

  1 in total

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