Brevundimonas terrae sp. nov., isolated from an alkaline soil in Korea.

A Gram-negative, rod-shaped, Brevundimonas-like bacterial strain, KSL-145(T), was isolated from an alkaline soil in Korea and subjected to a polyphasic taxonomic investigation. Strain KSL-145(T) grew optimally at pH 7.5-8.0 and 30 degrees C without NaCl. It was characterized chemotaxonomically as containing Q-10 as the predominant ubiquinone and C(18 : 1)omega7c and C(16 : 0) as the major fatty acids. The DNA G+C content was 61.8 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain KSL-145(T) fell within the radiation of the cluster comprising Brevundimonas species and Mycoplana bullata. The levels of 16S rRNA gene sequence similarity between strain KSL-145(T) and the type strains of Brevundimonas species and M. bullata ranged from 95.3 to 98.7 %. The mean DNA-DNA relatedness values between strain KSL-145(T) and the type strains of Brevundimonas diminuta and M. bullata, the closest phylogenetic relatives, were 26 and 15 %, respectively. Strain KSL-145(T) could be differentiated from Brevundimonas species and M. bullata by differences in several phenotypic characteristics. On the basis of the phenotypic, phylogenetic and genetic data, strain KSL-145(T) represents a novel species in the genus Brevundimonas, for which the name Brevundimonas terrae sp. nov. is proposed. The type strain is KSL-145(T) (=KCTC 12481(T)=JCM 13476(T)).