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Literature DB >> 17158571

Multi-nanopore force spectroscopy for DNA analysis.

Abstract

The need for low-cost DNA sequence detection in clinical applications is driving development of new technologies. We demonstrate a method for detection of mutations in a DNA sequence purely by electronic means, and without need for fluorescent labeling. Our method uses an array of nanopores to perform synchronized single-molecule force spectroscopy measurements over many molecules in parallel, yielding detailed information on the kinetics of hundreds of molecule dissociations in a single measurement.

Mesh：

Substances：
Oligonucleotides
Poly A

Year: 2006 PMID： 17158571 PMCID： PMC1796826 DOI： 10.1529/biophysj.106.094060

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

19 in total

Multi-nanopore force spectroscopy for DNA analysis.

1. Arrayed primer extension: solid-phase four-color DNA resequencing and mutation detection technology.

2. Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel.

Review 3. Probing the relation between force--lifetime--and chemistry in single molecular bonds.

4. Discrimination among individual Watson-Crick base pairs at the termini of single DNA hairpin molecules.

Review 5. Characterization of nucleic acids by nanopore analysis.

6. Protein biosensors based on biofunctionalized conical gold nanotubes.

7. Mutation detection by solid phase primer extension.

8. A nanosensor for transmembrane capture and identification of single nucleic Acid molecules.

9. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions.

Review 10. Nanometer-scale pores: potential applications for analyte detection and DNA characterization.

1. DNA translocation and unzipping through a nanopore: some geometrical effects.

2. Deciphering ionic current signatures of DNA transport through a nanopore.

Review 3. Single molecule sensing by nanopores and nanopore devices.

4. Exploring transmembrane transport through alpha-hemolysin with grid-steered molecular dynamics.

5. Enhanced translocation of single DNA molecules through alpha-hemolysin nanopores by manipulation of internal charge.

6. Conductance-Based Determination of Solid-State Nanopore Size and Shape: An Exploration of Performance Limits.

7. Probing DNA base pairing energy profiles using a nanopore.

8. Nonexponential kinetics of DNA escape from alpha-hemolysin nanopores.

9. Encapsulating a single G-quadruplex aptamer in a protein nanocavity.

10. Electro-osmotic screening of the DNA charge in a nanopore.