| Literature DB >> 17157399 |
Renata K T Kobayashi1, Luis Carlos J Gaziri, Emerson J Venancio, Marilda C Vidotto.
Abstract
The temperature-sensitive hemagglutinin (Tsh, 140 kDa) produced by Escherichia coli is cleaved into a fragment (106 kDa) containing mucinase activity, and an agglutinin fragment (33 kDa). By incorporating mucins into SDS-PAGE gels stained by Schiff's periodic acid, we could simultaneously detect about 0.5 microg of mucinase activity and the fragment molecular mass.Entities:
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Year: 2006 PMID： 17157399 DOI： 10.1016/j.mimet.2006.10.002
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363