| Literature DB >> 17157016 |
Ruel E McKnight1, Aaron B Gleason, James A Keyes, Sadia Sahabi.
Abstract
A topoisomerase I DNA unwinding assay has been used to determine the relative DNA-binding affinities of a model pair of homologous naphthalene diimides. Binding affinity data were corroborated using calorimetric (ITC) and spectrophotometric (titration and T(m)) studies, with substituent size playing a significant role in binding. The assay was also used to investigate the mode of binding adopted by several known DNA-binding agents, including SYBR Green and PicoGreen. Some of the compounds exhibited unexpected binding modes.Entities:
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Year: 2006 PMID： 17157016 DOI： 10.1016/j.bmcl.2006.11.038
Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823