BACKGROUND AND AIM OF THE STUDY: A new-generation heart valve was developed to improve long-term durability. The study aim was to evaluate the need for in-vitro autologous endothelial cell seeding of a decellularized valve scaffold. METHODS: A seeded (group I, n = 6) or non-seeded (group II, n=6) decellularized valve was implanted into the right ventricular outflow tract of 12 juvenile sheep. In group I, a jugular vein was harvested to characterize and expand endothelial cells (ECs). The scaffold was seeded in vitro, using a sedimentation technique. In group II, non-seeded decellularized scaffolds were implanted and explantation was performed at three and six months. Valves were evaluated by echocardiography, gross pathology, X-radiography, histology, and immunohistochemistry. RESULTS: In group I, the EC seeding density was 1.06 +/- 0.01 x 10(5) cells/cm2, with a cell viability of 95.7 +/- 1.4%. No regurgitation was visualized by echocardiography. Gross pathology showed smooth leaflets without retraction; calcification was absent at X-radiography, and this was confirmed by von Kossa staining. Histologically, group I valves showed a persistence of ECs, whereas a monolayer of host ECs was seen at six months in group II valves. Host fibroblasts were available in both groups, and numbers increased over time. Differences were identified in the recellularization density of in-vitro-seeded and non-seeded valves for up to three months, but no such difference was seen after six months. CONCLUSION: Based on results of studies in a sheep model, there appears to be no need for in-vitro cell seeding of decellularized scaffolds.
BACKGROUND AND AIM OF THE STUDY: A new-generation heart valve was developed to improve long-term durability. The study aim was to evaluate the need for in-vitro autologous endothelial cell seeding of a decellularized valve scaffold. METHODS: A seeded (group I, n = 6) or non-seeded (group II, n=6) decellularized valve was implanted into the right ventricular outflow tract of 12 juvenile sheep. In group I, a jugular vein was harvested to characterize and expand endothelial cells (ECs). The scaffold was seeded in vitro, using a sedimentation technique. In group II, non-seeded decellularized scaffolds were implanted and explantation was performed at three and six months. Valves were evaluated by echocardiography, gross pathology, X-radiography, histology, and immunohistochemistry. RESULTS: In group I, the EC seeding density was 1.06 +/- 0.01 x 10(5) cells/cm2, with a cell viability of 95.7 +/- 1.4%. No regurgitation was visualized by echocardiography. Gross pathology showed smooth leaflets without retraction; calcification was absent at X-radiography, and this was confirmed by von Kossa staining. Histologically, group I valves showed a persistence of ECs, whereas a monolayer of host ECs was seen at six months in group II valves. Host fibroblasts were available in both groups, and numbers increased over time. Differences were identified in the recellularization density of in-vitro-seeded and non-seeded valves for up to three months, but no such difference was seen after six months. CONCLUSION: Based on results of studies in a sheep model, there appears to be no need for in-vitro cell seeding of decellularized scaffolds.
Authors: José Rodolfo Paniagua Gutierrez; Helen Berry; Sotirios Korossis; Saeed Mirsadraee; Sergio Veiga Lopes; Francisco da Costa; John Kearney; Kevin Watterson; John Fisher; Eileen Ingham Journal: Tissue Eng Part A Date: 2014-10-01 Impact factor: 3.845
Authors: Tayyebeh Vafaee; Fiona Walker; Dan Thomas; João Gabriel Roderjan; Sergio Veiga Lopes; Francisco DA da Costa; Amisha Desai; Paul Rooney; Louise M Jennings; John Fisher; Helen E Berry; Eileen Ingham Journal: J Tissue Eng Date: 2022-06-28 Impact factor: 7.940
Authors: Anneke Neumann; Samir Sarikouch; Thomas Breymann; Serghei Cebotari; Dietmar Boethig; Alexander Horke; Philipp Beerbaum; Mechthild Westhoff-Bleck; Harald Bertram; Masamichi Ono; Igor Tudorache; Axel Haverich; Gernot Beutel Journal: Tissue Eng Part A Date: 2014-01-24 Impact factor: 3.845
Authors: Pascal Maria Dohmen; Francisco da Costa; Sergio Vega Lopes; Ricardo Vilani; Oliver Bloch; Wolfgang Konertz Journal: Med Sci Monit Basic Res Date: 2014-01-10