| Literature DB >> 17147984 |
Hao Cheng1, Zhongfa Liu, William Blum, John C Byrd, Rebecca Klisovic, Michael R Grever, Guido Marcucci, Kenneth K Chan.
Abstract
A specific and sensitive HPLC-MS/MS method for the quantitative determination of valproic acid (VPA) and its metabolite, 2-propyl-4-pentenoic acid in human plasma has been developed, using VPA-d15 as the internal standard. The method was based on pre-column derivatization using 4-dimethylaminobenzylamine dihydrochloride. The derivatives were separated with a gradient elution and quantified by positive electrospray ionization with multiple reaction monitoring. The assay provides routine quantification limits of 200 ng/mL for VPA and 20 ng/mL for 4-ene VPA with within- and between-day coefficients of variation of <10%. This method has been applied to the analysis of plasma samples obtained from patients treated with this drug.Entities:
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Year: 2006 PMID： 17147984 DOI： 10.1016/j.jchromb.2006.11.027
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205