Literature DB >> 17142916

Crystallization and preliminary X-ray crystallographic studies of the oxidative-stress sensor SoxR and its complex with DNA.

Satoshi Watanabe1, Akiko Kita, Kazuo Kobayashi, Yasuhiro Takahashi, Kunio Miki.   

Abstract

SoxR, a member of the MerR family of transcriptional activators, functions as a sensor of oxidative stress. The redox states of the 2Fe-2S cluster of SoxR regulate the activity of SoxR. Here, the crystallization and preliminary crystallographic analysis of SoxR and its complex with DNA are reported. Crystals of SoxR were obtained using PEG 10,000 and glycerol as precipitants. The crystals of SoxR belong to space group P6(2) or P6(4), with unit-cell parameters a = b = 80.0, c = 88.1 A. Crystals of the SoxR-DNA complex were obtained using a 20 bp DNA fragment from a condition containing PEG 10,000 and sodium/potassium tartrate. The crystals of the SoxR-DNA complex belong to space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 53.5, c = 355.6 A. Diffraction data were collected to a maximum resolution of 3.2 and 2.7 A for SoxR and the SoxR-DNA complex, respectively.

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Year:  2006        PMID： 17142916      PMCID： PMC2225378          DOI： 10.1107/S1744309106048482

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  29 in total

1.  SoxR, a [2Fe-2S] transcription factor, is active only in its oxidized form.

Authors:  P Gaudu; B Weiss
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-17       Impact factor: 11.205

2.  The redox state of the [2Fe-2S] clusters in SoxR protein regulates its activity as a transcription factor.

Authors:  H Ding; E Hidalgo; B Demple
Journal:  J Biol Chem       Date:  1996-12-27       Impact factor: 5.157

3.  Redox signal transduction: mutations shifting [2Fe-2S] centers of the SoxR sensor-regulator to the oxidized form.

Authors:  E Hidalgo; H Ding; B Demple
Journal:  Cell       Date:  1997-01-10       Impact factor: 41.582

4.  Spacing of promoter elements regulates the basal expression of the soxS gene and converts SoxR from a transcriptional activator into a repressor.

Authors:  E Hidalgo; B Demple
Journal:  EMBO J       Date:  1997-03-03       Impact factor: 11.598

5.  Overproduction and physical characterization of SoxR, a [2Fe-2S] protein that governs an oxidative response regulon in Escherichia coli.

Authors:  J Wu; W R Dunham; B Weiss
Journal:  J Biol Chem       Date:  1995-04-28       Impact factor: 5.157

6.  DNA-bend modulation in a repressor-to-activator switching mechanism.

Authors:  A Z Ansari; J E Bradner; T V O'Halloran
Journal:  Nature       Date:  1995-03-23       Impact factor: 49.962

7.  Isolation of reductase for SoxR that governs an oxidative response regulon from Escherichia coli.

Authors:  K Kobayashi; S Tagawa
Journal:  FEBS Lett       Date:  1999-05-28       Impact factor: 4.124

8.  Activation by nitric oxide of an oxidative-stress response that defends Escherichia coli against activated macrophages.

Authors:  T Nunoshiba; T deRojas-Walker; J S Wishnok; S R Tannenbaum; B Demple
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-01       Impact factor: 11.205

9.  Two-stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the soxS regulatory gene.

Authors:  T Nunoshiba; E Hidalgo; C F Amábile Cuevas; B Demple
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

10.  Binuclear [2Fe-2S] clusters in the Escherichia coli SoxR protein and role of the metal centers in transcription.

Authors:  E Hidalgo; J M Bollinger; T M Bradley; C T Walsh; B Demple
Journal:  J Biol Chem       Date:  1995-09-08       Impact factor: 5.157
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  4 in total

1.  Crystal structure of the [2Fe-2S] oxidative-stress sensor SoxR bound to DNA.

Authors:  Satoshi Watanabe; Akiko Kita; Kazuo Kobayashi; Kunio Miki
Journal:  Proc Natl Acad Sci U S A       Date:  2008-03-11       Impact factor: 11.205

2.  Direct oxidation of the [2Fe-2S] cluster in SoxR protein by superoxide: distinct differential sensitivity to superoxide-mediated signal transduction.

Authors:  Mayu Fujikawa; Kazuo Kobayashi; Takahiro Kozawa
Journal:  J Biol Chem       Date:  2012-08-20       Impact factor: 5.157

3.  DNA binding shifts the redox potential of the transcription factor SoxR.

Authors:  Alon A Gorodetsky; Lars E P Dietrich; Paul E Lee; Bruce Demple; Dianne K Newman; Jacqueline K Barton
Journal:  Proc Natl Acad Sci U S A       Date:  2008-03-03       Impact factor: 11.205

4.  Identification of Position-Specific Correlations between DNA-Binding Domains and Their Binding Sites. Application to the MerR Family of Transcription Factors.

Authors:  Yuriy D Korostelev; Ilya A Zharov; Andrey A Mironov; Alexandra B Rakhmaininova; Mikhail S Gelfand
Journal:  PLoS One       Date:  2016-09-30       Impact factor: 3.240
  4 in total

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