| Literature DB >> 17141165 |
Marcia Ramos Monteiro da Silva1, Antônio Augusto Mendes Maia, Noeli Maria Espíndola, Luís dos Ramos Machado, Adelaide José Vaz, Flávio Henrique-Silva.
Abstract
In order to evaluate the potential use of TS14 antigen in an enzyme-linked immunosorbent assay (ELISA) for immunodiagnosis of neurocysticercosis (NC), its open reading frame (ORF) was amplified by RT-PCR from mRNA isolated from Taenia solium cysticerci. The ORF was subcloned into the expression vector pET-28a, and was used to transform Escherichia coli BL21 (DE3) cells to produce TS14 antigen. The His-tagged expressed protein was purified on a nickel affinity column. Using the HISTS14 as antigen, ELISA was positive for 100% of cerebrospinal fluid (CSF) and 97% of serum samples from NC patients. No positive results were observed with sera and CSF samples from control groups. Cross-reactivity with sera from patients with schistosomiasis and Chagas' disease was not observed. Serum samples from patients with taeniasis were evaluated and 2 of 13 cases showed reactivity in this assay. Our data indicate the usefulness of HISTS14 in ELISA for an accurate and rapid assay for diagnosis of NC and seroepidemiological studies.Entities:
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Year: 2006 PMID： 17141165 DOI： 10.1016/j.actatropica.2006.10.009
Source DB: PubMed Journal: Acta Trop ISSN： 0001-706X Impact factor: 3.112