| Literature DB >> 1713918 |
Abstract
Expression and yield in E. coli of a panel of fusion proteins containing various domains of Epstein-Barr virus nuclear antigens, EBNA-1, EBNA-2, EBNA-3, EBNA-4 and EBNA-6, were scrutinized. The antigenicity of the EBNA fusion proteins against human sera was examined. Monospecific antisera to the different EBNA domains were produced by immunizing guinea pigs and rabbits. An EBNA-6 fusion polypeptide was useful for separating anti-EBNA-6 antibody from human sera by immunoaffinity purification. The applications of the fusion proteins to clinical diagnosis are discussed.Entities:
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Year: 1991 PMID： 1713918 DOI： 10.1016/0166-0934(91)90168-y
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014