| Literature DB >> 17128267 |
Saskia Schröder-Lang1, Martin Schwärzel, Reinhard Seifert, Timo Strünker, Suneel Kateriya, Jens Looser, Masakatsu Watanabe, U Benjamin Kaupp, Peter Hegemann, Georg Nagel.
Abstract
The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACalpha and PACbeta. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.Entities:
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Year: 2006 PMID： 17128267 DOI： 10.1038/nmeth975
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547