| Literature DB >> 17126075 |
Luz M T Paz Maldonado1, Víctor E Balderas Hernández, Emilio Medina Rivero, Ana P Barba de la Rosa, José L Flores Flores, Leandro G Ordoñez Acevedo, Antonio De León Rodríguez.
Abstract
A human interferon beta (hINF-beta) synthetic gene was optimized and expressed in Escherichia coli BL21-SI using a vector with the T7 promoter. To determine the best culture conditions such as culture medium, temperature, cell density and inducer concentration, we used the response surface methodology and a Box-Behnken design to get the highest hINF-beta production. The maximum hINF-beta production of 61 mg l(-1) was attained using minimum medium and the following predicted optimal conditions: temperature of 32.5 degrees C, cell density of 0.64, and inducer concentration of 0.30 M NaCl. This is the first report showing the successful performance of the BL21-SI system in a minimum medium. The response surface methodology is effective for the optimization of recombinant protein production using synthetic genes.Entities:
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Year: 2006 PMID： 17126075 DOI： 10.1016/j.bioeng.2006.10.001
Source DB: PubMed Journal: Biomol Eng ISSN： 1389-0344