Literature DB >> 17122010

Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens.

Kwannan Nantavisai1, Mathirut Mungthin, Peerapan Tan-ariya, Ram Rangsin, Tawee Naaglor, Saovanee Leelayoova.   

Abstract

Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitivities and specificities.

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Year:  2006        PMID： 17122010      PMCID： PMC1829074          DOI： 10.1128/JCM.01823-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa.

Authors:  P A Orlandi; K A Lampel
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

2.  An unusually compact ribosomal DNA repeat in the protozoan Giardia lamblia.

Authors:  J C Boothroyd; A Wang; D A Campbell; C C Wang
Journal:  Nucleic Acids Res       Date:  1987-05-26       Impact factor: 16.971

3.  Prevalence and genotypes of Giardia duodenalis in post-weaned dairy calves.

Authors:  James M Trout; Mónica Santín; Ellis Greiner; Ronald Fayer
Journal:  Vet Parasitol       Date:  2005-04-25       Impact factor: 2.738

4.  Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality.

Authors:  R M Hopkins; B P Meloni; D M Groth; J D Wetherall; J A Reynoldson; R C Thompson
Journal:  J Parasitol       Date:  1997-02       Impact factor: 1.276

5.  Molecular systematics of the parasitic protozoan Giardia intestinalis.

Authors:  P T Monis; R H Andrews; G Mayrhofer; P L Ey
Journal:  Mol Biol Evol       Date:  1999-09       Impact factor: 16.240

6.  Epidemiological and molecular evidence supports the zoonotic transmission of Giardia among humans and dogs living in the same community.

Authors:  R J Traub; P T Monis; I Robertson; P Irwin; N Mencke; R C A Thompson
Journal:  Parasitology       Date:  2004-03       Impact factor: 3.234

7.  Evaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens.

Authors:  Stephanie P Johnston; Melissa M Ballard; Michael J Beach; Louise Causer; Patricia P Wilkins
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

Review 8.  Current trends in research into the waterborne parasite Giardia.

Authors:  Samantha Lane; David Lloyd
Journal:  Crit Rev Microbiol       Date:  2002       Impact factor: 7.624

9.  Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP.

Authors:  Carolyn M Read; Paul T Monis; R C Andrew Thompson
Journal:  Infect Genet Evol       Date:  2004-06       Impact factor: 3.342

10.  Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis.

Authors:  Irshad M Sulaiman; Ronald Fayer; Caryn Bern; Robert H Gilman; James M Trout; Peter M Schantz; Pradeep Das; Altaf A Lal; Lihua Xiao
Journal:  Emerg Infect Dis       Date:  2003-11       Impact factor: 6.883
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  12 in total

1.  DNA extraction methods and multiple sampling to improve molecular diagnosis of Sarcocystis spp. in cattle hearts.

Authors:  Patrícia Bräunig; Luiza Pires Portella; Alfredo Skrebsky Cezar; Felipe Libardoni; Luis Antonio Sangioni; Fernanda Silveira Flores Vogel; Paulo Bayard Dias Gonçalves
Journal:  Parasitol Res       Date:  2016-06-08       Impact factor: 2.289

2.  Comparison and evaluation of four methods for extracting DNA from Giardia duodenalis cysts for PCR targeting the tpi gene.

Authors:  Akram Sepahvand; Nader Pestehchian; Hossein Ali Yousefi; Reza Pahlavan Gharehbaba
Journal:  J Parasit Dis       Date:  2016-06-10

3.  Is "dried stool spots on filter paper method (DSSFP)" more sensitive and effective for detecting Blastocystis spp. and their subtypes by PCR and sequencing?

Authors:  Ayse Seyer; Djursun Karasartova; Emrah Ruh; Ayse Semra Güreser; Turgut Imir; Aysegul Taylan-Ozkan
Journal:  Parasitol Res       Date:  2016-08-17       Impact factor: 2.289

4.  Evaluation of a PCR protocol for sensitive detection of Giardia intestinalis in human faeces.

Authors:  Amy J Asher; Liette S Waldron; Michelle L Power
Journal:  Parasitol Res       Date:  2011-07-29       Impact factor: 2.289

5.  Evaluation of the zoonotic potential of Giardia duodenalis in fecal samples from dogs and cats in Ontario.

Authors:  Rebeccah M McDowall; Andrew S Peregrine; Erin K Leonard; Christopher Lacombe; Mary Lake; Ana R Rebelo; Hugh Y Cai
Journal:  Can Vet J       Date:  2011-12       Impact factor: 1.008

6.  Molecular epidemiology of Cryptosporidium spp. and Giardia spp. in mussels (Mytilus californianus) and California sea lions (Zalophus californianus) from Central California.

Authors:  A D Adell; W A Smith; K Shapiro; A Melli; P A Conrad
Journal:  Appl Environ Microbiol       Date:  2014-10-03       Impact factor: 4.792

7.  Imported malaria in United Arab Emirates: evaluation of a new DNA extraction technique using nested PCR.

Authors:  Doaa M Sultan; Marwa M Khalil; Ahmed S Abdouh; Wafaa F Doleh; Abdul Aziz M Al Muthanna
Journal:  Korean J Parasitol       Date:  2009-08-28       Impact factor: 1.341

8.  Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois.

Authors:  Claudio De Liberato; Federica Berrilli; Marianna Marangi; Maristella Santoro; Tiziana Trogu; Lorenza Putignani; Paolo Lanfranchi; Francesco Ferretti; Stefano D'Amelio; Annunziata Giangaspero
Journal:  Parasit Vectors       Date:  2015-12-21       Impact factor: 3.876

9.  Real-time PCR: Benefits for Detection of Mild and Asymptomatic Giardia Infections.

Authors:  Rapeepun Prasertbun; Yaowalark Sukthana; Supaluk Popruk
Journal:  Trop Med Health       Date:  2012-06-27

10.  Molecular Identification of Giardia duodenalis Isolates from Fars Province, Iran.

Authors:  Mohammad Rayani; Ngah Zasmy Unyah; Gholamreza Hatam
Journal:  Iran J Parasitol       Date:  2014-03       Impact factor: 1.012
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