Literature DB >> 17121391

A semiautomatic instrument for the radioautographic coating technique.

B M Kopriwa1.   

Abstract

By means of a mechanical coating instrument a fast, simple method to coat specimens with liquid nuclear track emulsion has been devised for quantitative light and electron microscopic radioautography. In both cases, the section is mounted on a glass slide. After the vertically held slide has been immersed in the melted emulsion, the instrument withdraws it at a slow, constant speed. As a result, the specimen is coated with a thin, uniform emulsion layer composed of homogeneously distributed silver bromide crystals. The thickness of the emulsion coat may be standardized by selection of an optimal combination of emulsion dilution, temperature and withdrawal speed.

Entities:  

Mesh:

Substances:

Year:  1966        PMID: 17121391     DOI: 10.1177/14.12.923

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN: 0022-1554            Impact factor:   2.479


  26 in total

1.  Leaf structure and translocation in sugar beet.

Authors:  D R Geiger; D A Cataldo
Journal:  Plant Physiol       Date:  1969-01       Impact factor: 8.340

2.  The autoradiographical assoc-iation of fast transported material with dense core vesicles in the central nervous system of Anodonta cygnea (l).

Authors:  E A Howes; B J McLaughlin; J P Heslop
Journal:  Cell Tissue Res       Date:  1974       Impact factor: 5.249

3.  Function of coated membranes and multivesicular bodies during membrane regulation in stimulated exocrine pancreas cells.

Authors:  J J Geuze; M F Kramer
Journal:  Cell Tissue Res       Date:  1974       Impact factor: 5.249

4.  A reliable, standardized method for ultrastructural electron microscopic radioautography.

Authors:  B M Kopriwa
Journal:  Histochemie       Date:  1973-10-03

5.  Autoradiographic detection of 3 H-fucose incorporation into glycoprotein by odontoblasts and its deposition at the site of the calcification front in dentin.

Authors:  A Weinstock; M Weinstock; C P Leblond
Journal:  Calcif Tissue Res       Date:  1972

Review 6.  Matrix models. Essential tools for microscopic cytochemical research.

Authors:  M van der Ploeg; W A Duijndam
Journal:  Histochemistry       Date:  1986

7.  Enzyme layers on glass as a new model for the quantitative study of capture reactions in cytochemistry, with special attention to acid phosphatase.

Authors:  A S de Jong; T J Hak; M T Joe; P van Duijn
Journal:  Histochemistry       Date:  1978-09-28

8.  Autoradiographic study of tobramycin uptake by proximal and distal tubules of normal and pyelonephritic rats.

Authors:  M G Bergeron; Y Marois; C Kuehn; F J Silverblatt
Journal:  Antimicrob Agents Chemother       Date:  1987-09       Impact factor: 5.191

9.  Phenidone-ascorbic acid development in electronmicroscopic autoradiography.

Authors:  H Heijnen; H Geuze
Journal:  Histochemistry       Date:  1977-10-03

Review 10.  Mechanisms of metal--salt methods in enzyme cytochemistry with special reference to acid phosphatase.

Authors:  A S De Jong
Journal:  Histochem J       Date:  1982-01
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.