Literature DB >> 17120736

P53 regulation of leukemia cells with the blockage of MDM2 by antisense oligonucleotides.

Minghao Fang1, Xuemei Ji, Yi Tang, Wenli Liu.   

Abstract

The changes of expression and function of MDM2 and P53 by MDM2 specific antisense oligonucleotides were investigated in HL60 cells. Cells were divided into control group, AS group (MDM2 specific antisense oligonucleotides group), cisplatin group, and combined treatment group. FCM analysis and Western blot and RT-PCR were used to estimate apoptosis and the expression of MDM2 and P53. Our results showed that the transfection of MDM2 specific antisense oligonucleotides obviously inhibited MDM2 expression (P < 0.01) and increased the expression of P53 (P < 0.05). Apoptosis rate were reduced by MDM2 specific antisense oligonucletides and cisplatin (P < 0.01). It is concluded that MDM2 specific antisense oligonucletides can inhibit the expression of MDM2, induce the expression of P53 and increase the apoptosis of leukemia cells after chemotherapy.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 17120736     DOI： 10.1007/s11596-006-0409-7

Source DB:  PubMed          Journal:  J Huazhong Univ Sci Technolog Med Sci        ISSN： 1672-0733


  7 in total

Review 1.  Intrasteric regulation of MDM2.

Authors:  Harumi Shimizu; Ted R Hupp
Journal:  Trends Biochem Sci       Date:  2003-07       Impact factor: 13.807

2.  Stabilization of the MDM2 oncoprotein by mutant p53.

Authors:  Y Peng; L Chen; C Li; W Lu; S Agrawal; J Chen
Journal:  J Biol Chem       Date:  2001-01-10       Impact factor: 5.157

3.  The p53-mdm-2 autoregulatory feedback loop.

Authors:  X Wu; J H Bayle; D Olson; A J Levine
Journal:  Genes Dev       Date:  1993-07       Impact factor: 11.361

4.  Participation of p53 protein in the cellular response to DNA damage.

Authors:  M B Kastan; O Onyekwere; D Sidransky; B Vogelstein; R W Craig
Journal:  Cancer Res       Date:  1991-12-01       Impact factor: 12.701

5.  Antisense MDM2 oligonucleotides restore the apoptotic response of prostate cancer cells to androgen deprivation.

Authors:  Zhaomei Mu; Paul Hachem; Sudhir Agrawal; Alan Pollack
Journal:  Prostate       Date:  2004-08-01       Impact factor: 4.104

6.  In vivo activation of the p53 pathway by small-molecule antagonists of MDM2.

Authors:  Lyubomir T Vassilev; Binh T Vu; Bradford Graves; Daisy Carvajal; Frank Podlaski; Zoran Filipovic; Norman Kong; Ursula Kammlott; Christine Lukacs; Christian Klein; Nader Fotouhi; Emily A Liu
Journal:  Science       Date:  2004-01-02       Impact factor: 47.728

7.  Antisense therapy targeting MDM2 oncogene in prostate cancer: Effects on proliferation, apoptosis, multiple gene expression, and chemotherapy.

Authors:  Zhuo Zhang; Mao Li; Hui Wang; Sudhir Agrawal; Ruiwen Zhang
Journal:  Proc Natl Acad Sci U S A       Date:  2003-09-16       Impact factor: 11.205
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.